Caffeic Acid Phenethyl Ester (CAPE) Induces VEGF Expression and Production in Rat Odontoblastic Cells

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書誌事項

タイトル
Caffeic Acid Phenethyl Ester (CAPE) Induces VEGF Expression and Production in Rat Odontoblastic Cells
タイトル別名
  • カフェイン酸フェネチルエステル（CAPE）がラット象牙芽細胞様細胞のVEGF発現と産生に与える影響
著者
蔵本, 瞳
著者別名
  • クラモト, ヒトミ
  • Kuramoto, Hitomi
著者
平尾, 功治
著者別名
  • ヒラオ, コウジ
  • Hirao, Kouji
著者
湯本, 浩通
著者別名
  • ユモト, ヒロミチ
  • Yumoto, Hiromichi
著者
細川, 由樹
著者別名
  • ホソカワ, ユキ
  • Hosokawa, Yuki
著者
中西, 正
著者別名
  • ナカニシ, タダシ
  • Nakanishi, Tadashi
著者
武川, 大輔
著者別名
  • タケガワ, ダイスケ
  • Takegawa, Daisuke
著者
ワシオ, アヤコ
著者別名
  • Washio, Ayako
著者
キタムラ, チアキ
著者別名
  • Kitamura, Chiaki
著者
松尾, 敬志
著者別名
  • マツオ, タカシ
  • Matsuo, Takashi
学位授与大学
徳島大学
取得学位
博士（歯学）
学位授与番号
甲口第455号
学位授与年月日
2020-03-23

説明

Caffeic acid phenethyl ester (CAPE), the main component of propolis, has various biological activities including anti-inflammatory effect and wound healing promotion. Odontoblasts located in the outermost layer of dental pulp play crucial roles such as production of growth factors and formation of hard tissue termed reparative dentin in host defense against dental caries. In this study, we investigated the effects of CAPE on the upregulation of vascular endothelial growth factor (VEGF) and calcification activities of odontoblasts, leading to development of novel therapy for dental pulp inflammation caused by dental caries. CAPE significantly induced mRNA expression and production of VEGF in rat clonal odontoblast-like KN-3 cells cultured in normal medium or osteogenic induction medium. CAPE treatment enhanced nuclear factor-kappa B (NF-κB) transcription factor activation, and furthermore, the specific inhibitor of NF-κB significantly reduced VEGF production. The expression of VEGF receptor- (VEGFR-) 2, not VEGFR-1, was up regulated in KN-3 cells treated with CAPE. In addition, VEGF significantly increased mineralization activity in KN-3 cells. These findings suggest that CAPE might be useful as a novel biological material for the dental pulp conservative therapy.

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