A Novel Method of DAPI Staining for Differential Diagnosis of Renal Amyloidosis
書誌事項
- タイトル
- A Novel Method of DAPI Staining for Differential Diagnosis of Renal Amyloidosis
- タイトル別名
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- DAPI染色による腎アミロイドーシスの新しい鑑別方法
- Diagnosis of renal AL amyloidosis using DAPI
- 著者
- 松浦, 元一
- 著者別名
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- マツウラ, モトカズ
- Matsuura, Motokazu
- 著者
- 安部, 秀斉
- 著者別名
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- アベ, ヒデハル
- Abe, Hideharu
- 著者
- 冨永, 辰也
- 著者別名
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- トミナガ, タツヤ
- Tominaga, Tatsuya
- 著者
- 櫻井, 明子
- 著者別名
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- サクライ, アキコ
- Sakurai, Akiko
- 著者
- 村上, 太一
- 著者別名
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- ムラカミ, タイチ
- Murakami, Taichi
- 著者
- 岸, 誠司
- 著者別名
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- キシ, セイジ
- Kishi, Seiji
- 著者
- 坂東, 良美
- 著者別名
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- バンドウ, ヨシミ
- Bando, Yoshimi
- 著者
- ミナクチ, ジュン
- 著者別名
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- Minakuchi, Jun
- 著者
- 長井, 幸二郎
- 著者別名
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- ナガイ, コウジロウ
- Nagai, Kojiro
- 著者
- 土井, 俊夫
- 著者別名
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- ドイ, トシオ
- Doi, Toshio
- 学位授与大学
- 徳島大学
- 取得学位
- 博士（医学）
- 学位授与番号
- 乙医第1753号
- 学位授与年月日
- 2017-11-30
説明
Amyloidosis is often overlooked because its clinical manifestations can mimic those of more-common diseases. It is important to get a precise diagnosis as early as possible for the prevention of further organ damages. Amyloidosis is a disorder caused by deposition of insoluble abnormal amyloid. The kidney is a frequent site of amyloid deposition. The amyloid fibrils have a characteristic appearance and generate birefringence under polarized light when stained with the Congo red dye. Classification of amyloidosis is based on the precursor protein that forms the amyloid fibrils and the distribution of amyloid deposits as either systemic or localized. Involvement of amyloid fibrils in kidneys mainly occurs as amyloid light-chain (AL) or amyloid A (AA) amyloidosis. The potassium permanganate method with Congo red staining was once used widely to discriminate AL and AA amyloidoses, but thismethod has a problem of false positive results. We found that extracellular and cytoplasmic glomerular 4’,6-diamidino-2-phenylindole (DAPI)-positive areas were clearly consistent with amyloid deposition in AL amyloidosis. In contrast, the overlapping staining was not seen in AA amyloidosis. Therefore, we propose that DAPI staining readily distinguishes AL renal amyloidosis from AA renal amyloidosis as a simple and reproducible histochemical method.
目次
2019-09-06 再収集
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詳細情報 詳細情報について
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- CRID
- 1910585658881907200
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- NII論文ID
- 500001621248
- 500001051432
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- 本文言語コード
- en
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- データソース種別
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- IRDB
- NDLサーチ