Effect of quercetin and its metabolite on caveolin-1 expression induced by oxidized LDL and lysophosphatidylcholine in endothelial cells
書誌事項
- タイトル
- Effect of quercetin and its metabolite on caveolin-1 expression induced by oxidized LDL and lysophosphatidylcholine in endothelial cells
- タイトル別名
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- 血管内皮細胞における酸化LDLおよびリゾホスファチジルコリン誘導によるカベオリン-1発現に対するケルセチンおよび代謝物の効果
- 著者
- 鎌田, 智英実
- 著者別名
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- カマダ, チエミ
- Kamada, Chiemi
- 著者
- 向井, 理恵
- 著者
- コンドウ, アカリ
- 著者別名
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- Kondo, Akari
- 著者
- サトウ, シンヤ
- 著者別名
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- Sato, Shinya
- 著者
- 寺尾, 純二
- 学位授与大学
- 徳島大学
- 取得学位
- 博士(栄養学)
- 学位授与番号
- 甲栄第233号
- 学位授与年月日
- 2016-03-23
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説明
Oxidized low-density lipoprotein contributes to atherosclerotic plaque formation, and quercetin is expected to exert anti-atherosclerotic effects. We previously reported accumulation of conjugated quercetin metabolites in the aorta of rabbits fed high-cholesterol diets with quercetin glucosides, resulting in attenuation of lipid peroxidation and inhibition of lipid accumulation. Caveolin-1, a major structural protein of caveolae in vascular endothelial cells, plays a role in atherosclerosis development. Here we investigated effects of oxidized low-density lipoprotein, quercetin and its metabolite, quercetin 3-O-β-glucuronide, on caveolin-1 expression. Oxidized low-density lipoprotein significantly upregulated caveolin-1 mRNA expression. An oxidized low-density lipoprotein component, lysophosphatidylcholine, also induced expression of both caveolin-1 mRNA and protein. However, lysophosphatidylcholine did not affect the location of caveolin-1 proteins within caveolae structures. Co-treatment with quercetin or quercetin 3-O-β-glucuronide inhibited lysophosphatidylcholine-induced caveolin-1 expression. Quercetin and quercetin 3-O-β-glucuronide also suppressed expression of adhesion molecules induced by oxidized low-density lipoprotein and lysophosphatidylcholine. These results strongly suggest lysophosphatidylcholine derived from oxidized low-density lipoprotein contributes to atherosclerotic events by upregulating caveolin-1 expression, resulting in induction of adhesion molecules. Quercetin metabolites are likely to exert an anti-atherosclerotic effect by attenuating caveolin-1 expression in endothelial cells.