Characterization of the short isoform of Helios overexpressed in patients with T-cell malignancies

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Title
Characterization of the short isoform of Helios overexpressed in patients with T-cell malignancies
Other Title
  • Ｔ細胞性造血器腫瘍において過剰発現が認められるHelios short isoformの機能解析
Author
多林, 孝之
University
岡山大学
Types of degree
博士（医学）
Grant ID
甲第3299号
Degree year
2007-03-23

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Description

In an earlier report, we demonstrated overexpression of a short isoform of Helios, Hel-5, which lacks three of four N-terminal zinc fingers, in patients with adult T-cell leukemia/lymphoma. Here, we characterized Hel-5 using immunoprecipitation, and gel shift and luciferase promoter assays, and found that Hel-5 lacks the repressor function observed with a full-length isoform of Helios. Moreover, Hel-5 associates with the full-length isoforms of the Ikaros gene family, Ikaros, Aiolos and Helios, and inhibits their DNA binding activity when present in excess, leading to dominant-negative effects on the full-length isoforms of the Ikaros gene family. Our results suggest a critical role for Helios in the mechanism of leukemogenesis.

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