UV Treatment Improves the Biocompatibility and Antibacterial Properties of Crystallized Nanostructured Titanium Surface

Previous reports have revealed that heat treatment is applied to pure titanium metal on which nanostructures have been deposited to crystallize the nanostructures. In this study, we examined the effect of UV treatment on the sample that was crystallized by heat treatment after nanostructured (TNS) of pure titanium metal by concentrated alkali treatment, and the effect on biocompatibility and antibacterial property. There were three experimental groups: a TNS group, a TNS group subjected to heat treatment, and a TNS group subjected to heat treatment and UV treatment. Surface analysis of various material surfaces by SEM, SPM, TF-XRD showed nano-level network structure and crystallization in heat treatment and heat treatment and UV treatment group and XPS analysis showed material surface in UV treatment group A remarkable decrease in the peak of C indicating staining was observed. In addition, it was confirmed by the measurement of the contact angle that the decrease of the C peak induced the improvement of the wettability of the material surface. In experiments using rat bone marrow cells and human umbilical cord vascular endothelial cells, it was shown that improving the initial adhesion of various cells and the ability to induce hard tissue differentiation showed the highest values ​​by UV treatment and heat treatment, and Staphylococcus aureus was used. Bacterial experiments showed that the biofilm removal rate and staining test showed strong antibacterial properties. In the in vivo evaluation of implanting each treated implant into the rat femur, micro-CT analysis of the rat femur cross-section including the implant revealed new hard tissue formation around the implant. The most observed in UV treatment and heat treatment. Histological observation also revealed that the amount of hard tissue formed was significantly higher in the UV-treated and heat-treated groups, indicating that the material was highly biocompatible. From the above results, it is possible to impart hydrophilicity and antibacterial property by subjecting a pure titanium metal having a nanostructure deposited to a heat treatment and subjecting a material having a nanostructure to a UV treatment to UV treatment. It became clear both in vitro and in vivo.

2019年度

収集根拠 : 博士論文（自動収集）
資料形態 : テキストデータ
コレクション : 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文
Previous reports have revealed that heat treatment is applied to pure titanium metal on which nanostructures have been deposited to crystallize the nanostructures. In this study, we examined the effect of UV treatment on the sample that was crystallized by heat treatment after nanostructured (TNS) of pure titanium metal by concentrated alkali treatment, and the effect on biocompatibility and antibacterial property. There were three experimental groups: a TNS group, a TNS group subjected to heat treatment, and a TNS group subjected to heat treatment and UV treatment. Surface analysis of various material surfaces by SEM, SPM, TF-XRD showed nano-level network structure and crystallization in heat treatment and heat treatment and UV treatment group and XPS analysis showed material surface in UV treatment group A remarkable decrease in the peak of C indicating staining was observed. In addition, it was confirmed by the measurement of the contact angle that the decrease of the C peak induced the improvement of the wettability of the material surface. In experiments using rat bone marrow cells and human umbilical cord vascular endothelial cells, it was shown that improving the initial adhesion of various cells and the ability to induce hard tissue differentiation showed the highest values ​​by UV treatment and heat treatment, and Staphylococcus aureus was used. Bacterial experiments showed that the biofilm removal rate and staining test showed strong antibacterial properties. In the in vivo evaluation of implanting each treated implant into the rat femur, micro-CT analysis of the rat femur cross-section including the implant revealed new hard tissue formation around the implant. The most observed in UV treatment and heat treatment. Histological observation also revealed that the amount of hard tissue formed was significantly higher in the UV-treated and heat-treated groups, indicating that the material was highly biocompatible. From the above results, it is possible to impart hydrophilicity and antibacterial property by subjecting a pure titanium metal having a nanostructure deposited to a heat treatment and subjecting a material having a nanostructure to a UV treatment to UV treatment. It became clear both in vitro and in vivo.
2019年度