Campylobacter jejuni infection suppressed Cl⁻ secretion induced by CFTR activation in T-84 cells

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Bibliographic Information

Title
Campylobacter jejuni infection suppressed Cl⁻ secretion induced by CFTR activation in T-84 cells
Other Title
  • Campylobacter jejuni感染はT-84細胞におけるCFTR活性化によるCl⁻分泌亢進を抑制する
Author
Negoro, Sachie
Alias Name
  • 天野, 幸恵
  • アマノ, サチエ
Author
Shimohata, Takaaki
Alias Name
  • 下畑, 隆明
  • シモハタ, タカアキ
Author
Hatayama, Syo
Alias Name
  • ハタヤマ, ショウ
  • ハタヤマ, ショウ
Author
Sato, Yuri
Alias Name
  • サトウ, ユリ
  • サトウ, ユリ
Author
Matsumoto, Mari
Alias Name
  • マツモト, マリ
  • マツモト, マリ
Author
Iba, Hitomi
Alias Name
  • イバ, ヒトミ
  • イバ, ヒトミ
Author
Nakahashi-Aihara, Mutsumi
Alias Name
  • (中橋)粟飯原, 睦美
  • (ナカハシ)アイハラ, ムツミ
Author
Uebansou, Takashi
Alias Name
  • 上番増, 喬
  • ウエバンソウ, タカシ
Author
Hamada, Yasuhiro
Alias Name
  • 濵田, 康弘
  • ハマダ, ヤスヒロ
Author
Nishikawa, Yoshikazu
Alias Name
  • ニシカワ, ヨシカズ
  • ニシカワ, ヨシカズ
Author
Yamasaki, Shinji
Alias Name
  • ヤマサキ, シンジ
  • ヤマサキ, シンジ
Author
Mawatari, Kazuaki
Alias Name
  • 馬渡, 一諭
  • マワタリ, カズアキ
Author
Takahashi, Akira
Alias Name
  • 髙橋, 章
  • タカハシ, アキラ
University
徳島大学
Types of degree
博士（栄養学）
Grant ID
甲栄第241号
Degree year
2017-03-23

Description

Campylobacter jejuni causes foodborne disease associated with abdominal pain, gastroenteritis, and diarrhea. These symptoms are induced by bacterial adherence and invasion of host epithelial cells. C. jejuni infection can occur with a low infective dose, suggesting that C. jejuni may have evolved strategies to cope with the bacterial clearance system in the gastrointestinal tract. The mucosa layer is the first line of defense against bacteria. Mucus conditions are maintained by water and anion (especially Cl-) movement. Cystic fibrosis transmembrane conductance regulator (CFTR) is the main Cl- channel transporting Cl- to the lumen. Mutations in CFTR result in dehydrated secreted mucus and bacterial accumulation in the lungs, and recent studies suggest that closely related pathogenic bacteria also may survive in the intestine. However, the relationship between C. jejuni infection and CFTR has been little studied. Here, we used an 125I- efflux assay and measurement of short-circuit current to measure Cl- secretion in C. jejuni-infected T-84 human intestinal epithelial cells. The basic state of Cl- secretion was unchanged by C. jejuni infection, but CFTR activator was observed to induce Cl- secretion suppressed in C. jejuniinfected T-84 cells. The suppression of activated Cl- secretion was bacterial dose-dependent and duration-dependent. A similar result was observed during infection with other C. jejuni strains. The mechanism of suppression may occur by affecting water movement or mucus condition in the intestinal tract. A failure of mucus barrier function may promote bacterial adhesion or invasion of host intestinal epithelial cells, thereby causing bacterial preservation in the host intestinal tract.

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