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- 吉川 祐子
- IPU・環太平洋大学次世代教育学部
書誌事項
- タイトル別名
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- Real-Time Visualization of Double-Strands Break in DNA : Quantitative Analysis on the Protective Effect of Vitamin C
- DNA 2ジュウサ セツダン ハンノウ ノ カシカ ビタミン C ニ ヨル ヨクセイ コウカ ノ テイリョウテキ ケイソク
この論文をさがす
抄録
Direct attack to genomic DNA by reactive oxygen species causes various types of lesions. The most significant lesion is considered to be an unrepaired double-strand break that can lead to fatal cell damage. Using fluorescence microscopy, we directly observed double-strand breaks of DNA in reconstituted chromatin stained by a fluorescent cyanine dye, YOYO, in solution. YOYO is known to have the ability to photo-cleave DNAs by generating reactive oxygen species. We also investigated the effect of an antioxidant vitamin, vitamin C, on preventing double-strand breaks in a quantitative manner. We showed that doublestrand breaks can be monitored at the level of individual DNA molecules, and that the kinetics of the breakage reaction can be deduced from such single-molecule observation. We found that DNA is protected against double-strand breaks by the addition of vitamin C.
収録刊行物
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- ビタミン
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ビタミン 81 (7), 297-302, 2007
公益社団法人 日本ビタミン学会
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詳細情報 詳細情報について
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- CRID
- 1390282680676805120
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- NII論文ID
- 110006368520
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- NII書誌ID
- AN00207833
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- ISSN
- 2424080X
- 0006386X
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- NDL書誌ID
- 8902181
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- NDL
- CiNii Articles
- KAKEN
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- 抄録ライセンスフラグ
- 使用不可