Development and applications of next-generation epigenomics technologies

KAKEN

About This Project

Japan Grant Number
JP17H06305 (JGN)
Funding Program
Grants-in-Aid for Scientific Research
Funding Organization
Japan Society for the Promotion of Science

Kakenhi Information

Project/Area Number
17H06305
Research Category
Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
Allocation Type
  • Single-year Grants
Review Section / Research Field
  • Biological Sciences
Research Institution
  • Kyushu University
Project Period (FY)
2017-06-30 〜 2022-03-31
Project Status
Completed
Budget Amount*help
127,270,000 Yen (Direct Cost: 97,900,000 Yen Indirect Cost: 29,370,000 Yen)

Research Abstract

In this project, we aimed to develop highly-sensitive and/or multiplexed epigenome sequencing methods, which should be critical to accelerate trans-omic analyses. First, we developed a highly efficient method for single-stranded DNA ligation termed TACS ligation and introduced it to further improve the performance of PBAT, the most sensitive and reliable methylome sequencing method of our own. Second, we developed DMS-seq for in vivo genome-wide mapping of protein-DNA interactions and nucleosome centers, thus adding a unique method to the toolbox for epigenomics. Third, we applied TACS ligation to cell-free DNA analysis and revealed a novel class of short single-stranded DNA enriched with noncanonical DNA structure, or antisense of G-quadruplex structure. Fourth, we identified a novel DNA methyltransferase, which should lay a basis for a multiplexed epigenomics method to encode histone modification information to methylation of neighborhood DNA.

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