Development of precision induction of organoids based on biomaterial design of fluidic and curved extracellular matrix
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- Kidoaki Satoru
- Principal Investigator
- 九州大学
About this project
- Japan Grant Number
- JP18H04167
- Funding Program
- Grants-in-Aid for Scientific Research
- Funding organization
- Japan Society for the Promotion of Science
- Project/Area Number
- 18H04167
- Research Category
- Grant-in-Aid for Scientific Research (A)
- Allocation Type
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- Single-year Grants
- Review Section / Research Field
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- Medium-sized Section 90:Biomedical engineering and related fields
- Research Institution
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- Kyushu University
- Project Period (FY)
- 2018-04-01 〜 2022-03-31
- Project Status
- Completed
- Budget Amount*help
- 44,460,000 Yen (Direct Cost: 34,200,000 Yen Indirect Cost: 10,260,000 Yen)
Research Abstract
To develop a technique and basic understandings for high reproducible and efficient preparation of organoids, i.e., the budding structures of artificial small organs formed in a culture environment, we systematically investigated the effects of parameters of viscoelastic properties and shape-inducing curvature of scaffolds for organoid culture. The rheological properties of Matrigel, which is commonly used in conventional organoid culture, were modulated by additional cross-linking and mechanical fragmentation of the matrix to determine the optimal viscoelastic property of the matrix for organoid formation. A simple synthetic polymer, PEG, was physically crosslinked with modified polypeptides, and the determined optimal viscoelastic properties were introduced to a laminin-loaded system through regulating the strength of physical crosslinking. This synthetic polymer matrix successfully produced liver organoids more stable than in the Matrigel system.
Details 詳細情報について
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- CRID
- 1040000781994357888
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- Text Lang
- ja
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- Data Source
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- KAKEN
