Establishment of the the method to develop 3D-periodontal ligament from iPS cells, and its application for artificial tooth root
About this project
- Japan Grant Number
- JP17H01598
- Funding Program
- Grants-in-Aid for Scientific Research
- Funding organization
- Japan Society for the Promotion of Science
- Project/Area Number
- 17H01598
- Research Category
- Grant-in-Aid for Scientific Research (A)
- Allocation Type
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- Single-year Grants
- Review Section / Research Field
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- Biological Sciences > Medicine, Dentistry, and Pharmacy > Dentistry > Conservative dentistry
- Research Institution
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- Kyushu University
- Project Period (FY)
- 2017-04-01 〜 2022-03-31
- Project Status
- Completed
- Budget Amount*help
- 42,640,000 Yen (Direct Cost: 32,800,000 Yen Indirect Cost: 9,840,000 Yen)
Research Abstract
We clarified the method to induce periodontal ligament (PDL) stem cell-like cells (iPDLSC) from iPS cells, and identified the transcription factor, PAX9, and Fibrillin 2 as the factors to induce the differentiation of iPS cells to iPDLSC. We also developed the tube-like structure from the spheroids derived from human PDL stem-like cell line that we produced. This structure exhibited not only the PDL-like properties, but the features of cementum, blood vessels, and bone by inserting a hydroxyapatite (HAp) cylinder into the structure. Finally, we prepared the tube-like structure made of iPDLSC spheroids, and observed the engraftment of this structure including the HAp cylinder.
Keywords
Details 詳細情報について
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- CRID
- 1040282256931296000
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- Text Lang
- ja
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- Data Source
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- KAKEN
- IRDB
