Analyses of stem cell genomes in long-lived plants
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- Satake Akiko
- Principal Investigator
- 九州大学
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- 陶山 佳久
- Co-Investigator
- 東北大学
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- 谷 尚樹
- Co-Investigator
- 国立研究開発法人国際農林水産業研究センター
About this project
- Japan Grant Number
- JP17H06478
- Funding Program
- Grants-in-Aid for Scientific Research
- Funding organization
- Japan Society for the Promotion of Science
- Project/Area Number
- 17H06478
- Research Category
- Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
- Allocation Type
-
- Single-year Grants
- Review Section / Research Field
-
- Biological Sciences
- Research Institution
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- Kyushu University
- Project Period (FY)
- 2017-06-30 〜 2022-03-31
- Project Status
- Completed
- Budget Amount*help
- 72,670,000 Yen (Direct Cost: 55,900,000 Yen Indirect Cost: 16,770,000 Yen)
Research Abstract
To determine whether stem cell genomes are maintained stably in long-lived organisms, they generated a high-quality genome of Shorea laevis and detected somatic mutations that accumulated over 400 years to accurately estimate the mutation rate prior to natural selection and genetic drift in the next generation population. The number of somatic mutations was shown to increase linearly with growth, and the rate of de novo mutations per base per year was accurately estimated. Detailed analysis of the mutation spectrum showed that mutations caused by DNA damage to UV light were the most abundant (53.1%). Comparative genomic analysis suggested that trees have a higher copy number of poly(ADP-ribose) polymerase (PARP) than perennial and annual plants, contributing to their long-term survival and protection from DNA damage and pathogen infection. These results provide important knowledge in understanding the evolution of DNA repair and lifespan in plants.