Omics approaches towards the elucidation of the molecular network regulating the developmental capacity of the mammalian oocyte
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- SASAKI Hiroyuki
- Principal Investigator
- 九州大学
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- 丸山 修
- Co-Investigator
- 九州大学
About this project
- Japan Grant Number
- JP18H05214
- Funding Program
- Grants-in-Aid for Scientific Research
- Funding organization
- Japan Society for the Promotion of Science
- Project/Area Number
- 18H05214
- Research Category
- Grant-in-Aid for Specially Promoted Research
- Allocation Type
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- Single-year Grants
- Review Section / Research Field
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- Biological Sciences
- Research Institution
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- Kyushu University
- Project Period (FY)
- 2018-04-23 〜 2023-03-31
- Project Status
- Completed
- Budget Amount*help
- 508,560,000 Yen (Direct Cost: 391,200,000 Yen Indirect Cost: 117,360,000 Yen)
Research Abstract
By applying genome editing and small-scale omics approaches, we have revealed the molecular network regulating histone modifications and DNA methylation in mouse oocytes, which are essential for mammalian development. We discovered that several histone modifications impact the efficiency and distribution of DNA methylation and identified the proteins and their domains that mediate the epigenetic crosstalk. Further, we found that, while DNA methylation established in oocytes regulates the expression of the maternally imprinted genes almost exclusively in embryos, histone modifications and their responsive factors have a wider effect and regulate various cellular function including chromosome segregation. Finally, we developed a machine-learning-based model that predicts the heritability of DNA methylation to the next generation based on DNA sequence. The findings provide a basis for the study of infertility, various diseases, and improvement of assisted reproductive technologies.
Details 詳細情報について
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- CRID
- 1040282256971482112
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- Text Lang
- ja
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- Data Source
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- KAKEN