Optimization of stomatal regulation that enables both high CO2 absorption and drought tolarance
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- Kusumi Kensuke
- Principal Investigator
- 九州大学
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- 祢宜 淳太郎
- Co-Investigator
- 九州大学
About this project
- Japan Grant Number
- JP18K06294
- Funding Program
- Grants-in-Aid for Scientific Research
- Funding organization
- Japan Society for the Promotion of Science
- Project/Area Number
- 18K06294
- Research Category
- Grant-in-Aid for Scientific Research (C)
- Allocation Type
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- Multi-year Fund
- Review Section / Research Field
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- Basic Section 44030:Plant molecular biology and physiology-related
- Research Institution
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- Kyushu University
- Project Period (FY)
- 2018-04-01 〜 2022-03-31
- Project Status
- Completed
- Budget Amount*help
- 4,290,000 Yen (Direct Cost: 3,300,000 Yen Indirect Cost: 990,000 Yen)
Research Abstract
To elucidate the regulatory mechanism of the balance between CO2 absorption and water transpiration through stomatal closure, we structurally modified rice SLAC1 protein, a key factor in the regulation of stomatal closure, by homologous recombination-mediated genome editing. We succeeded in introducing two amino acid substitutions (Y256F/Y473F) on the SLAC1 gene that disrupt the candidate CO2 signal receiving site. Gas exchange measurement exhibited that in the SLAC1-modified plants, elevated CO2-induced stomatal closure was significantly reduced. By contrast, ABA-induced stomatal closure remained unaffected. These results indicate that stomatal CO2 response can be artificially controlled by modification of the SLAC1 structure.
Details 詳細情報について
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- CRID
- 1040282256976278656
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- Text Lang
- ja
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- Data Source
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- KAKEN