Exploration of the mechanism(s) of cartilage degeneration in osteoarthritis through the analysis of exosomes in the synovial fluid.

  • Tsuno Hirotaka
    Principal Investigator
    独立行政法人国立病院機構(相模原病院臨床研究センター)

About this project

Project/Area Number
18K16638
Research Category
Grant-in-Aid for Early-Career Scientists
Allocation Type
  • Multi-year Fund
Review Section / Research Field
  • Basic Section 56020:Orthopedics-related
Research Institution
  • Clinical Research Center for Allergy and Rheumatology, National Hospital Organization, Sagamihara National Hospital
Project Period (FY)
2018-04-01 〜 2021-03-31
Project Status
Completed
Budget Amount*help
4,160,000 Yen (Direct Cost: 3,200,000 Yen Indirect Cost: 960,000 Yen)
Funding Program
Grants-in-Aid for Scientific Research

Research Abstract

Although pain is the most critical symptom of osteoarthritis (OA). Recent epidemiological studies have shown that the synovial pathology is closely related to pain in OA knees. However, the mechanisms underlying synovial pathology in OA has not yet been clarified, and the same is true for the reasons why pain is associated with the synovial pathology. In this study, we focused on miRNA involved in the pain in OA knees. To that end, synovial fluid (SF) samples were collected from OA knees twice at the peak of pain and after the remission of pain, and exosomes were obtained from these SF samples. miRNA was extracted from the exosomes and the miRNAs related to pain were analyzed by qPCR analyses. The results were compared between the SF samples from respective knees, and miRNA was determined the concentrations of which differed significantly between the two points of time. In results, a total of 5 miRNAs were found which could be related to pain in OA knee joints.

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