In Vitro Antimicrobial Activity of Green Synthesized Silver Nanoparticles Against Selected Gram-negative Foodborne Pathogens

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  • 西渕, 光昭
    Department of Food Science, Faculty of Food Science and Technology, Universiti Putra Malaysia
  • Rukayadi, Yaya
    Department of Food Science, Faculty of Food Science and Technology, Universiti Putra Malaysia
  • Nor-Khaizura, Mahmud-Ab-Rashid
    Department of Food Science, Faculty of Food Science and Technology, Universiti Putra Malaysia
  • Kuan, Chee Hao
    Department of Agricultural and Food Science, Faculty of Science, Universiti Tunku Abdul Rahman
  • Chieng, Buong Woei
    Department of Chemistry, Faculty of Science, Universiti Putra Malaysia･Materials Processing and Technology Laboratory, Institute of Advanced Technology, Universiti Putra Malaysia
  • Nishibuchi, Mitsuaki
    Center for Southeast Asian Studies, Kyoto University
  • Radu, Son
    Department of Food Science, Faculty of Food Science and Technology･Laboratory of Food Safety and Food Integrity, Institute of Tropical Agriculture and Food Security (ITAFoS), Universiti Putra Malaysia

抄録

Silver nanoparticles (AgNPs) used in this study were synthesized using pu-erh tea leaves extract with particle size of 4.06 nm. The antibacterial activity of green synthesized AgNPs against a diverse range of Gram-negative foodborne pathogens was determined using disk diffusion method, resazurin microtitre-plate assay (minimum inhibitory concentration, MIC), and minimum bactericidal concentration test (MBC). The MIC and MBC of AgNPs against Escherichia coli, Klebsiella pneumoniae, Salmonella Typhimurium, and Salmonella Enteritidis were 7.8, 3.9, 3.9, 3.9 and 7.8, 3.9, 7.8, 3.9 μg/mL, respectively. Time-kill curves were used to evaluate the concentration between MIC and bactericidal activity of AgNPs at concentrations ranging from 0×MIC to 8×MIC. The killing activity of AgNPs was fast acting against all the Gram-negative bacteria tested; the reduction in the number of CFU mL⁻¹ was >3 Log₁₀ units (99.9%) in 1–2 h. This study indicates that AgNPs exhibit a strong antimicrobial activity and thus might be developed as a new type of antimicrobial agents for the treatment of bacterial infection including multidrug resistant bacterial infection.

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詳細情報 詳細情報について

  • CRID
    1050282813185968384
  • NII論文ID
    120006554788
  • ISSN
    1664302X
  • HANDLE
    2433/236376
  • 本文言語コード
    en
  • 資料種別
    journal article
  • データソース種別
    • IRDB
    • CiNii Articles

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