Direct observation of dynamic force propagation between focal adhesions of cells on microposts by atomic force microscopy

Okada, Akinori, Mizutani, Yusuke, Subagyo, Agus, Hosoi, Hirotaka, Nakamura, Motonori, Sueoka, Kazuhisa, Kawahara, Koichi, Okajima, Takaharu

doi:10.1063/1.3672225

Description

We investigated dynamic force propagation between focal adhesions of fibroblast cells cultured on polydimethylsiloxane micropost substrates, by atomic force microscopy. Live cells were mechanically modulated by the atomic force microscopy probe bound to cell apical surfaces at 0.01-0.5 Hz, while microposts served as a force sensor at basal surfaces. We observed that cells exhibited rheological behavior at the apical surface but had no apparent out-of-phase response at the basal surface, indicating that the dynamic force propagating through cytoskeletal filaments behaves in an elastic manner. Moreover, the direction of the propagated force was observed to be intimately associated with the prestress.

Journal

Applied Physics Letters

Applied Physics Letters 99 (26), 263703-, 2011-12-26

American Institute of Physics

Keywords

Details 詳細情報について

CRID: 1050282813987259392

NII Article ID: 120003783352

DOI: 10.1063/1.3672225

ISSN: 10773118; 00036951

HANDLE: 2115/48293

Web Site: https://pubs.aip.org/aip/apl/article-pdf/doi/10.1063/1.3672225/14463435/263703_1_online.pdf

Text Lang: en

Article Type: journal article

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