書誌事項
- タイトル別名
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- コウジョウセン ホルモン オウトウ ハイレツ オ クミ カエタ レポータープラスミド オ モチイタ イデンシ カッセイ シケン システム ニ ツイテ
- Gene Activation Assay System Using Reporter Plasmid with Thyroid Hormone Responsive Element
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説明
RXR/TR reporter plasmid for gene activation assay was produced using recombinant DNA techniques. Thyroid hormone responsive element nucleotide sequences (AGGTTC tttcAGGTTC) was inserted into a reporter plasmid (pGL3 vector). After transfection of RXR/TR reporter plasmid into HepG2 cells, the activities of luciferin-luciferase were determined on various concentrations of thyroid hormone (T3). This reporter plasmid gene activation assay was highly sensitive to allow the detection of T3 effects in HepG2 cell, detecting the effect of 10-8 to 10-4 M thyroid hormone. Further experiments using the RXR/TR reporter plasmid gene activation assay system will be conducted in order to detect of thyroid hormone like-compounds in environmental pollutants.
収録刊行物
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- 北海道立衛生研究所報 = Report of the Hokkaido Institute of Public Health
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北海道立衛生研究所報 = Report of the Hokkaido Institute of Public Health 53 9-13, 2003-10-31
北海道立衛生研究所
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詳細情報 詳細情報について
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- CRID
- 1050283688373854848
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- NII論文ID
- 120006788124
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- NII書誌ID
- AN00231077
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- ISSN
- 04410793
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- NDL書誌ID
- 6802238
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- 本文言語コード
- ja
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- 資料種別
- journal article
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- データソース種別
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- IRDB
- NDL
- CiNii Articles