A novel strategy to facilitate uniform epithelial cell maturation using liquid–liquid interfaces

HANDLE Open Access
  • Sonoi, Rie
    Department of Chemical Engineering, Faculty of Engineering, Kyushu University
  • Kamihira, Masamichi
    Department of Chemical Engineering, Faculty of Engineering, Kyushu University

Description

Epithelial tissue forms and maintains a critical barrier function in the body. A novel culture design aimed at promoting uniform maturation of epithelial cells using liquid materials is described. Culturing Madin–Darby canine kidney (MDCK) cells at the liquid–liquid interface yielded reduced migration and stimulated active cell growth. Similar to solid–liquid interfaces, cells cultured on a fibronectin-coated liquid–liquid interface exhibited active migration and growth, ultimately reaching a confluent state. These cells exhibited reduced stress fiber formation and adopted a cobblestone-like shape, which led to their even distribution in the culture vessel. To inhibit stress fiber formation and apoptosis, the exposure of cells on liquid–liquid interfaces to Y27632, a specific inhibitor of the Rho-associated protein kinase (ROCK), facilitated tight junction formation (frequency of ZO-2-positive cells, F_Z = 0.73). In Y27632-exposed cells on the liquid–liquid interface, the value obtained by subtracting the standard deviation of the ratio of nucleus densities in each region that compartmentalized a culture vessel from 1, denoted as H_<LN>, was 0.93 ± 0.01, indicated even cell distribution in the culture vessel at t = 72 h. The behavior of epithelial cells on liquid–liquid interfaces contributes to the promotion of their uniform maturation.

Journal

Related Projects

See more

Details 詳細情報について

  • CRID
    1050581854677866624
  • ISSN
    20452322
  • HANDLE
    2324/7178836
  • Text Lang
    en
  • Article Type
    journal article
  • Data Source
    • IRDB

Report a problem

Back to top