A novel strategy to facilitate uniform epithelial cell maturation using liquid–liquid interfaces

DOI DOI DOI DOI 機関リポジトリ (HANDLE) ほか2件をすべて表示 一部だけ表示 被引用文献1件 参考文献30件 オープンアクセス

書誌事項

公開日
2024-05-29
資源種別
journal article
権利情報
  • Creative Commons Attribution 4.0 International
  • © The Author(s) 2024
DOI
  • 10.1038/s41598-024-63115-7
  • 10.21203/rs.3.rs-3993493/v1
  • 10.21203/rs.3.rs-3926366/v3
  • 10.21203/rs.3.rs-3926366/v2
公開者
Springer

説明

Epithelial tissue forms and maintains a critical barrier function in the body. A novel culture design aimed at promoting uniform maturation of epithelial cells using liquid materials is described. Culturing Madin–Darby canine kidney (MDCK) cells at the liquid–liquid interface yielded reduced migration and stimulated active cell growth. Similar to solid–liquid interfaces, cells cultured on a fibronectin-coated liquid–liquid interface exhibited active migration and growth, ultimately reaching a confluent state. These cells exhibited reduced stress fiber formation and adopted a cobblestone-like shape, which led to their even distribution in the culture vessel. To inhibit stress fiber formation and apoptosis, the exposure of cells on liquid–liquid interfaces to Y27632, a specific inhibitor of the Rho-associated protein kinase (ROCK), facilitated tight junction formation (frequency of ZO-2-positive cells, F_Z = 0.73). In Y27632-exposed cells on the liquid–liquid interface, the value obtained by subtracting the standard deviation of the ratio of nucleus densities in each region that compartmentalized a culture vessel from 1, denoted as H_<LN>, was 0.93 ± 0.01, indicated even cell distribution in the culture vessel at t = 72 h. The behavior of epithelial cells on liquid–liquid interfaces contributes to the promotion of their uniform maturation.

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