Wide-range screening of anti-inflammatory compounds in tomato using LC-MS and elucidating the mechanism of their functions.

HANDLE オープンアクセス
  • 毛利, 晋輔
    Laboratory of Molecular Function of Food, Graduate School of Agriculture, Kyoto University
  • 高橋, 春弥
    Laboratory of Molecular Function of Food, Graduate School of Agriculture, Kyoto University・KAGOME Tomato Discoveries Laboratory, Graduate School of Agriculture, Kyoto University
  • 高橋, 慎吾
    Laboratory of Molecular Function of Food, Graduate School of Agriculture, Kyoto University
  • 脇, 尚子
    Innovation Division, KAGOME CO., LTD.
  • 荒, 武
    KAGOME Tomato Discoveries Laboratory, Graduate School of Agriculture, Kyoto University・ Innovation Division, KAGOME CO., LTD.
  • 松村, 康生
    Innovation Division, KAGOME CO., LTD.
  • 柴田, 大輔
    Innovation Division, KAGOME CO., LTD.
  • 後藤, 剛
    KAGOME Tomato Discoveries Laboratory, Graduate School of Agriculture, Kyoto University
  • 河田, 照雄
    Laboratory of Quality Analysis and Assessment, Graduate School of Agriculture, Kyoto University
  • D, Shibata
    KAGOME Tomato Discoveries Laboratory, Graduate School of Agriculture, Kyoto University・Kazusa DNA Research Institutes, Kazusa-Kamatari
  • T, Goto
    Laboratory of Molecular Function of Food, Graduate School of Agriculture, Kyoto University・Research Unit for Physiological Chemistry, Kyoto University
  • T, Kawada
    Laboratory of Molecular Function of Food, Graduate School of Agriculture, Kyoto University・Research Unit for Physiological Chemistry, Kyoto University

抄録

Obesity-induced chronic inflammation is a key factor in type 2 diabetes. A vicious cycle involving pro-inflammatory mediators between adipocytes and macrophages is a common cause of chronic inflammation in the adipose tissue. Tomato is one of the most popular vegetables and is associated with a reduced risk of diabetes. However, the molecular mechanism underlying the effect of tomato on diabetes is unclear. In this study, we focused on anti-inflammatory compounds in tomato. We found that the extract of tomato reduced plasma glucose and inflammatory markers in mice. We screened anti-inflammatory fractions in tomato using lipopolysaccharide-stimulated RAW264.7 macrophages, and active compounds were estimated by liquid chromatography-mass spectrometry over a wide range. Surprisingly, a large number of compounds including oxylipin and coumarin derivatives were estimated as anti-inflammatory compounds. Especially, 9-oxo-octadecadienoic acid and daphnetin suppressed pro-inflammatory cytokines in RAW264.7 macrophages inhibiting mitogen-activated protein kinase phosphorylation and inhibitor of kappa B α protein degradation. These findings suggest that tomato containing diverse anti-inflammatory compounds ameliorates chronic inflammation in obese adipose tissue.

収録刊行物

  • PloS one

    PloS one 13 (1), 2018-01-12

    Public Library of Science (PLoS)

詳細情報 詳細情報について

  • CRID
    1050845760787544448
  • NII論文ID
    120006459972
  • ISSN
    19326203
  • HANDLE
    2433/230702
  • 本文言語コード
    en
  • 資料種別
    journal article
  • データソース種別
    • IRDB
    • CiNii Articles

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