神経病原性レトロウイルスA8-Vのenv遺伝子発現に見られた奇妙なスプライシング

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タイトル別名
  • シンケイ ビョウゲンセイ レトロウイルス Ａ８ － Ｖ ノ ｅｎｖ イデンシ ハツゲン ニ ミラレタ キミョウナ スプライシング
  • shinkei byogensei retoroirusu A8 - V no env idenshi hatsugen ni mirareta kimyona supuraishingu
  • Eccentric splicing found in env gene expression of neuropathogenic A8-V

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Packaging cell lines PacNIH/A8β and PacNIH/A8δ, which were introduced by the DNA of expression vectors pA8(Ψ-)βand pA8(Ψ-)δ ｒe spective1y, to produce pseud-retroviral particles in the retroviral vector system, were established. Both the expression vectors were constructed using the genes of A8-V, which is an isolate from Friend murine leukemia virus(F-MLV) and shows higher proliferation rates in the central nervous system(CNS) of rats and mice compared with original F-MLV. These two expression vectors utilize a different polyadenylation signal (polyA signal) for the expression of retroviral genes. The former employs a 0.85kb fragment in the SV40 early region as a polyA signal, and the latter, a 0.13kb of the same region. PacNIH/A8β showed an unstable expression of the env gene in the A8-V genome, whereas all of the retroviral gene expressions in the PacNIH/A8δ were perfect. An occurrence of abnormal splicing in the mRNA for the env gene in the PacNIH/A8β cells was suggested.

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収録刊行物

  • 慶應医学

    慶應医学 83 (1), 29-35, 2006-03

    慶應医学会

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