Involvement of luxS in Biofilm Formation by Capnocytophaga ochracea
書誌事項
- 公開日
- 2016-01-22
- 資源種別
- journal article
- 権利情報
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- ©2016 Hosohama-Saito et al. This is an open access article distributed under the terms of the Creative Commons Attribution License(http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
- DOI
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- 10.1371/journal.pone.0147114
- 公開者
- Public Library of Science
説明
Capnocytophaga ochracea is present in the dental plaque biofilm of patients with periodontitis. Biofilm cells change their phenotype through quorum sensing in response to fluctuations in cell-population density. Quorum sensing is mediated by auto-inducers (AIs). AI-2 is involved in intercellular signaling, and production of its distant precursor is catalyzed by LuxS, an enzyme involved in the activated methyl cycle. Our aim was to clarify the role of LuxS in biofilm formation by C. ochracea. Two luxS-deficient mutants, TmAI2 and LKT7, were constructed from C. ochracea ATCC 27872 by homologous recombination. The mutants produced significantly less AI-2 than the wild type. The growth rates of these mutants were similar to that of the wild-type in both undiluted Tryptic soy broth and 0.5 × Tryptic soy broth. However, according to crystal violet staining, they produced significantly less biofilm than the wild type. Confocal laser scanning microscopy and scanning electron microscopy showed that the biofilm of the TmAI2 strain had a rougher structure than that of the wild type. Complementation of TmAI-2 with extrinsic AI-2 from the culture supernatant of wild-type strain did not restore biofilm formation by the TmAI2 strain, but complementation of LKT7 strain with luxS partially restored biofilm formation. These results indicate that LuxS is involved in biofilm formation by C. ochracea, and that the attenuation of biofilm formation by the mutants is likely caused by a defect in the activated methyl cycle rather than by a loss of AI-2.
収録刊行物
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- PloS one
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PloS one 11 (1), e0147114-, 2016-01-22
Public Library of Science
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キーワード
詳細情報 詳細情報について
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- CRID
- 1050845762642397440
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- NII論文ID
- 120005853753
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- ISSN
- 19326203
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- HANDLE
- 10130/4076
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- PubMed
- 26800339
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- 本文言語コード
- en
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- 資料種別
- journal article
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- データソース種別
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