5′-Terminal stem-loop of carnation ringspot virus RNA1 is required for the efficient amplification of viral RNAs
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- 海道, 真典
- Laboratory of Plant Pathology, Graduate School of Agriculture, Kyoto University
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- 永野, 秀昭
- Laboratory of Plant Pathology, Graduate School of Agriculture, Kyoto University
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- 表, 和範
- Laboratory of Plant Pathology, Graduate School of Agriculture, Kyoto University
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- 髙野, 義孝
- Laboratory of Plant Pathology, Graduate School of Agriculture, Kyoto University
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- 三瀬, 和之
- Laboratory of Plant Pathology, Graduate School of Agriculture, Kyoto University
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- 奥野, 哲郎
- Laboratory of Plant Pathology, Graduate School of Agriculture, Kyoto University
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説明
Carnation ringspot virus (CRSV) is the prototype virus of the genus Dianthovirus. Full-length cDNAs of CRSV strainsPV-0097 and PV-21 were constructed and the infectivity of in vitro transcripts was analyzed. Infectivity of PV-0097 and PV-21 to several plants was markedly higher than that of 1.30, a previously reported infectious CRSV clone. Overall RNA sequences of these viruses were similar, but PV-0097 and PV-21 contained additional nucleotides at the 5′ end of RNA1. Stem-loop structures were predicted in the 5′-terminal region of PV-0097 and PV-21 RNA1 but not in 1.30 RNA1. Mutant CRSV 1.30 RNA1 that contains the terminal 4 nucleotides of PV-0097, predicted to fold a 5′-terminal stem-loop structure, recovered higher level accumulation of viral RNAs in the inoculated protoplasts and leaves of Nicotiana benthamiana. These results suggest that the 5′-terminal stem-loop structure of CRSV RNA1 plays an important role in efficient amplification of the virus.
収録刊行物
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- Virus Research
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Virus Research 265 138-142, 2019-05
Elsevier B.V.
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詳細情報 詳細情報について
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- CRID
- 1050845763174846592
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- NII論文ID
- 120006621537
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- ISSN
- 01681702
- 18727492
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- HANDLE
- 2433/240991
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- PubMed
- 30890436
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- 本文言語コード
- en
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- 資料種別
- journal article
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- データソース種別
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