Vascular endothelial growth factor receptor-1 (VEGFR-1) signaling enhances angiogenesis in a surgical sponge model.
書誌事項
- 公開日
- 2016-03
- 資源種別
- journal article
- 権利情報
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- https://www.elsevier.com/tdm/userlicense/1.0/
- https://www.elsevier.com/legal/tdmrep-license
- DOI
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- 10.1016/j.biopha.2016.01.005
- 10.1016/j.ajpath.2016.02.014
- 公開者
- Elsevier BV
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説明
The persistence of proinflammatory macrophages, which are recruited to the granulation tissue, impairs the healing of diabetic wounds. Herein, we examined the role of vascular endothelial growth factor receptor type 1 (VEGFR1) signaling in streptozotocin (STZ)-induced diabetic wound healing. Angiogenesis, lymphangiogenesis, and the healing of full-thickness skin wounds were impaired in STZ-treated wild-type (WT) mice compared with vehicle-treated WT mice, with attenuated recruitment of VEGFR1-positive macrophages expressing vascular endothelial growth factor (VEGF)-A, VEGF-C, and VEGF-D to the wound granulation tissue. These phenomena were even more prevalent in STZ-treated VEGFR1 tyrosine kinase knockout mice (VEGFR1 TK(-/-) mice). STZ-treated WT mice, but not STZ-treated VEGFR1 TK(-/-) mice, showed accelerated wound healing when treated with placenta growth factor. Compared with that of STZ-treated WT mice, the wound granulation tissue of STZ-treated VEGFR1 TK(-/-) mice contained more VEGFR1-positive cells expressing IL-1β [a classic (M1) activated macrophage marker] and fewer VEGFR1-positive cells expressing the mannose receptor [CD206; an alternatively activated (M2) macrophage marker]. Treatment of STZ-treated VEGFR1 TK(-/-) mice with an IL-1β-neutralizing antibody restored impaired wound healing and angiogenesis/lymphangiogenesis and induced macrophages in the wound granulation tissue to switch to an M2 phenotype. Taken together, these results suggest that VEGFR1 signaling plays a role in regulating the balance between macrophage phenotypes in STZ-induced diabetic wounds, prevents impaired diabetic wound healing, and promotes angiogenesis/lymphangiogenesis.
収録刊行物
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- Biomedicine & Pharmacotherapy
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Biomedicine & Pharmacotherapy 78 140-149, 2016-03
Elsevier BV
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キーワード
- Male
- Mice, Knockout
- Wound Healing
- Vascular Endothelial Growth Factor Receptor-1
- Macrophages
- Interleukin-1beta
- Fluorescent Antibody Technique
- Neovascularization, Physiologic
- Flow Cytometry
- Real-Time Polymerase Chain Reaction
- Cell Line
- Diabetes Mellitus, Experimental
- Mice, Inbred C57BL
- Mice
- Animals
- Humans
- Lymphangiogenesis
- Signal Transduction
詳細情報 詳細情報について
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- CRID
- 1360002215827703680
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- ISSN
- 07533322
- 00029440
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- Web Site
- https://api.elsevier.com/content/article/PII:S0753332215304376?httpAccept=text/xml
- https://api.elsevier.com/content/article/PII:S0753332215304376?httpAccept=text/plain
- https://api.elsevier.com/content/article/PII:S000294401630030X?httpAccept=text/xml
- https://api.elsevier.com/content/article/PII:S000294401630030X?httpAccept=text/plain
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- 資料種別
- journal article
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- データソース種別
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- Crossref
- KAKEN
- OpenAIRE
