<i>In Vivo</i> Imaging of the Intra- and Extracellular Redox Status in Rat Stomach with Indomethacin-Induced Gastric Ulcers Using Overhauser-Enhanced Magnetic Resonance Imaging
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- Keiji Yasukawa
- Laboratory of Advanced Pharmacology, Daiichi University of Pharmacy, Fukuoka, Japan.
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- Ryota Shigemi
- Physical Chemistry for Life Science Laboratory, Faculty of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan.
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- Tomomi Kanbe
- Physical Chemistry for Life Science Laboratory, Faculty of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan.
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- Yusaku Mutsumoto
- Physical Chemistry for Life Science Laboratory, Faculty of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan.
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- Fumiko Oda
- Physical Chemistry for Life Science Laboratory, Faculty of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan.
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- Kazuhiro Ichikawa
- Innovation Center for Medical Redox Navigation, Kyushu University, Fukuoka, Japan.
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- Ken-Ichi Yamada
- Physical Chemistry for Life Science Laboratory, Faculty of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan.
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- Xin Tun
- Division of Host Defense, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan.
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- Hideo Utsumi
- School of Pharmaceutical Sciences, University of Shizuoka, Shizuoka, Japan.
書誌事項
- 公開日
- 2019-03-20
- 資源種別
- journal article
- 権利情報
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- https://www.liebertpub.com/nv/resources-tools/text-and-data-mining-policy/121/
- DOI
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- 10.1089/ars.2017.7336
- 公開者
- SAGE Publications
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説明
Repeated use of nonsteroidal anti-inflammatory drugs can induce changes in the redox status, including production of reactive oxygen species (ROS), but the specific details of these changes remain unknown. Overhauser-enhanced magnetic resonance imaging (OMRI) has been used in vivo to monitor the redox status in several diseases and map tissue oxygen concentrations. We monitored the intra- and extracellular redox status in the stomach of rats with indomethacin-induced gastric ulcers using OMRI and investigated the relationship with gastric mucosal damage.One hour after oral administration of indomethacin (30 mg/kg), OMRI measurements in the stomach were made following nitroxyl probe administration. OMRI with the membrane-permeable nitroxyl probe, 4-hydroxy-2,2,6,6-tetramethyl-piperidine-1-oxyl (TEMPOL), demonstrated a redox change toward oxidation, which was reversed by a membrane-permeable antioxidant. Conversely, imaging with the impermeable probe, 4-trimethylammonium-2,2,6,6-tetramethyl-piperidine-1-oxyl (CAT-1), demonstrated little redox change. Redox imbalance imaging of a live rat stomach with indomethacin-induced gastric ulcers was produced by dual imaging ofOMRI combined with a dual probe is a less invasive imaging technique for evaluation of intracellular ROS production contributing to the formation of gastric ulcers in the stomach of indomethacin-treated rats, which cannot be done with other methods.This method may be a very powerful tool for characterizing the pathogenesis of various diseases and may have medical applications.
収録刊行物
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- Antioxidants & Redox Signaling
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Antioxidants & Redox Signaling 30 (9), 1147-1161, 2019-03-20
SAGE Publications
