<i>p</i>-Hydroxybenzoyl-Glucose Is a Zwitter Donor for the Biosynthesis of 7-Polyacylated Anthocyanin in Delphinium
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- Yuzo Nishizaki
- Department of Biotechnology and Life Science, Faculty of Engineering, Tokyo University of Agriculture and Technology, Koganei, Tokyo 184-8588, Japan
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- Motoki Yasunaga
- Department of Biotechnology and Life Science, Faculty of Engineering, Tokyo University of Agriculture and Technology, Koganei, Tokyo 184-8588, Japan
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- Emi Okamoto
- Department of Biotechnology and Life Science, Faculty of Engineering, Tokyo University of Agriculture and Technology, Koganei, Tokyo 184-8588, Japan
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- Mitsutoshi Okamoto
- Department of Agricultural Research, Ehime Research Institute of Agriculture, Forestry and Fisheries, Matsuyama, Ehime 799-2405, Japan
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- Yukio Hirose
- Department of Agricultural Research, Ehime Research Institute of Agriculture, Forestry and Fisheries, Matsuyama, Ehime 799-2405, Japan
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- Masaatsu Yamaguchi
- Faculty of Environmental Horticulture, Minami Kyushu University, Miyakonojo, Miyazaki 885-0035, Japan
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- Yoshihiro Ozeki
- Department of Biotechnology and Life Science, Faculty of Engineering, Tokyo University of Agriculture and Technology, Koganei, Tokyo 184-8588, Japan
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- Nobuhiro Sasaki
- Department of Biotechnology and Life Science, Faculty of Engineering, Tokyo University of Agriculture and Technology, Koganei, Tokyo 184-8588, Japan
書誌事項
- 公開日
- 2013-10
- 資源種別
- journal article
- 権利情報
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- http://aspb.org/publications/aspb-journals/open-articles
- DOI
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- 10.1105/tpc.113.113167
- 公開者
- Oxford University Press (OUP)
この論文をさがす
説明
The blue color of delphinium (Delphinium grandiflorum) flowers is produced by two 7-polyacylated anthocyanins, violdelphin and cyanodelphin. Violdelphin is derived from the chromophore delphinidin that has been modified at the 7-position by Glc and p-hydroxybenzoic acid (pHBA) molecules. Modification of violdelphin by linear conjugation of Glc and pHBA molecules to a Glc moiety at the 7-position produces cyanodelphin. We recently showed that anthocyanin 7-O-glucosylation in delphinium is catalyzed by the acyl-Glc-dependent anthocyanin glucosyltransferase (AAGT). Here, we sought to answer the question of which enzyme activities are necessary for catalyzing the transfer of Glc and pHBA moieties to 7-glucosylated anthocyanin. We found that these transfers were catalyzed by enzymes that use p-hydroxybenzoyl-Glc (pHBG) as a bifunctional acyl and glucosyl donor. In addition, we determined that violdelphin is synthesized via step-by-step enzymatic reactions catalyzed by two enzymes that use pHBG as an acyl or glucosyl donor. We also isolated a cDNA encoding a protein that has the potential for p-hydroxybenzoylation activity and two AAGT cDNAs that encode a protein capable of adding Glc to delphinidin 3-O-rutinoside-7-O-(6-O-[p-hydroxybenzoyl]-glucoside) to form violdelphin.
収録刊行物
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- The Plant Cell
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The Plant Cell 25 (10), 4150-4165, 2013-10
Oxford University Press (OUP)
