miR-1 and miR-206 regulate angiogenesis by modulating VegfA expression in zebrafish
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- Carlos Stahlhut
- Department of Genetics, Yale University School of Medicine, New Haven, CT 06510, USA
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- Yajaira Suárez
- Division of Cardiology/Department of Medicine, New York University School of Medicine, New York, NY 10016, USA
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- Jun Lu
- Yale Stem Cell Center, Yale University School of Medicine, New Haven, CT 06520, USA
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- Yuichiro Mishima
- Department of Genetics, Yale University School of Medicine, New Haven, CT 06510, USA
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- Antonio J. Giraldez
- Department of Genetics, Yale University School of Medicine, New Haven, CT 06510, USA
説明
<jats:p>Cellular communication across tissues is an essential process during embryonic development. Secreted factors with potent morphogenetic activity are key elements of this cross-talk, and precise regulation of their expression is required to elicit appropriate physiological responses. MicroRNAs (miRNAs) are versatile post-transcriptional modulators of gene expression. However, the large number of putative targets for each miRNA hinders the identification of physiologically relevant miRNA-target interactions. Here we show that miR-1 and miR-206 negatively regulate angiogenesis during zebrafish development. Using target protectors, our results indicate that miR-1/206 directly regulate the levels of Vascular endothelial growth factor A (VegfA) in muscle, controlling the strength of angiogenic signaling to the endothelium. Conversely, reducing the levels of VegfAa, but not VegfAb, rescued the increase in angiogenesis observed when miR-1/206 were knocked down. These findings uncover a novel function for miR-1/206 in the control of developmental angiogenesis through the regulation of VegfA, and identify a key role for miRNAs as regulators of cross-tissue signaling.</jats:p>
収録刊行物
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- Development
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Development 139 (23), 4356-4365, 2012-12-01
The Company of Biologists
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詳細情報 詳細情報について
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- CRID
- 1360002219091601408
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- ISSN
- 14779129
- 09501991
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- データソース種別
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- Crossref
- KAKEN