Metabolic engineering of <i>Corynebacterium glutamicum</i> for production of sunscreen shinorine
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- Yota Tsuge
- Graduate School of Natural Science and Technology, Kanazawa University , Kanazawa, Japan
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- Hideo Kawaguchi
- Graduate School of Science, Technology and Innovation, Kobe University , Kobe, Japan
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- Shogo Yamamoto
- Nagase R&D Center, Nagase & Co., Ltd ., Kobe, Japan
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- Yoshiko Nishigami
- Nagase R&D Center, Nagase & Co., Ltd ., Kobe, Japan
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- Masahiro Sota
- Nagase R&D Center, Nagase & Co., Ltd ., Kobe, Japan
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- Chiaki Ogino
- Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University , Kobe, Japan
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- Akihiko Kondo
- Graduate School of Science, Technology and Innovation, Kobe University , Kobe, Japan
書誌事項
- 公開日
- 2018-07-03
- 資源種別
- journal article
- 権利情報
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- https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model
- DOI
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- 10.1080/09168451.2018.1452602
- 10.6084/m9.figshare.6010589
- 10.6084/m9.figshare.6010589.v1
- 公開者
- Informa UK Limited
この論文をさがす
説明
<jats:title>Abstract</jats:title> <jats:p>Ultraviolet-absorbing chemicals are useful in cosmetics and skin care to prevent UV-induced skin damage. We demonstrate here that heterologous production of shinorine, which shows broad absorption maxima in the UV-A and UV-B region. A shinorine producing Corynebacterium glutamicum strain was constructed by expressing four genes from Actinosynnema mirum DSM 43827, which are responsible for the biosynthesis of shinorine from sedoheptulose-7-phosphate in the pentose phosphate pathway. Deletion of transaldolase encoding gene improved shinorine production by 5.2-fold. Among the other genes in pentose phosphate pathway, overexpression of 6-phosphogluconate dehydrogenase encoding gene further increased shinorine production by 60% (19.1 mg/L). The genetic engineering of the pentose phosphate pathway in C. glutamicum improved shinorine production by 8.3-fold in total, and could be applied to produce the other chemicals derived from sedoheptulose-7-phosphate.</jats:p>
収録刊行物
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 82 (7), 1252-1259, 2018-07-03
Informa UK Limited
