Copper induction of enhanced green fluorescent protein expression in<i>Pleurotus ostreatus</i>driven by laccase<i>poxa1b</i>promoter
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- Antonella Amore
- Department of Chemical Sciences; University of Naples ‘Federico II’; Complesso Universitario Monte S. Angelo; Napoli; Italy
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- Yoichi Honda
- Graduate School of Agriculture; Kyoto University; Kyoto; Japan
書誌事項
- 公開日
- 2012-10-29
- 資源種別
- journal article
- DOI
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- 10.1111/1574-6968.12023
- 公開者
- Oxford University Press (OUP)
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説明
In silico analyses of several laccase promoter sequences have shown the presence of many different responsive elements differentially distributed along the promoter sequences. Analysis of Pleurotus ostreatus laccase promoter poxa1b extending around 1400-bp upstream of the start codon showed the presence of several putative response elements, such as 10 metal-responsive elements. Development of a system for in vivo analysis of P. ostreatus laccase promoter poxa1b by enhanced green fluorescent protein expression was carried out, based on a polyethylene glycol-mediated procedure for fungal transformation. Quantitative measurement of fluorescence expressed in P. ostreatus transformants grown in the presence and in the absence of copper sulfate was performed, demonstrating an increase in expression level induced by the metal.
収録刊行物
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- FEMS Microbiology Letters
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FEMS Microbiology Letters 337 (2), 155-163, 2012-10-29
Oxford University Press (OUP)
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詳細情報 詳細情報について
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- CRID
- 1360004235458769664
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- HANDLE
- 11588/516620
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- ISSN
- 03781097
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- PubMed
- 23050832
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- 資料種別
- journal article
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- データソース種別
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- Crossref
- KAKEN
- OpenAIRE
