Interleukin‐6‐induced satellite cell proliferation is regulated by induction of the<scp>JAK</scp>2/<scp>STAT</scp>3 signalling pathway through cyclin D1 targeting
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- M. Kurosaka
- School of Physical Education Tokai University Kanagawa 259‐1292 Japan
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- S. Machida
- School of Physical Education Tokai University Kanagawa 259‐1292 Japan
書誌事項
- 公開日
- 2013-07-21
- 資源種別
- journal article
- 権利情報
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- http://onlinelibrary.wiley.com/termsAndConditions#vor
- DOI
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- 10.1111/cpr.12045
- 公開者
- Wiley
この論文をさがす
説明
<jats:title>Abstract</jats:title><jats:sec><jats:title>Objectives</jats:title><jats:p>To determine whether interleukin‐6 (<jats:styled-content style="fixed-case">IL</jats:styled-content>‐6) stimulates rat muscle satellite cell proliferation in culture, and if so, to clarify the signalling mechanisms.</jats:p></jats:sec><jats:sec><jats:title>Materials and methods</jats:title><jats:p>Primary satellite cells were isolated from thirty male F344 rats, 11 weeks of age.<jats:styled-content style="fixed-case">IL</jats:styled-content>‐6 at concentrations of 0.01, 0.1, 1, 10 or 100 ng/ml was added to culture media.</jats:p></jats:sec><jats:sec><jats:title>Results</jats:title><jats:p>IL‐6 at 0.01–1 ng/ml induced dose‐dependent increase in cell proliferation. After treatment with 1 ng/ml IL‐6, cell proliferation increased by 31%, and p‐STAT3<jats:sup>+</jats:sup>/MyoD<jats:sup>+</jats:sup>cells increased in number compared to those in control media (<jats:italic>P</jats:italic> < 0.05). Inhibitors of JAK2 (AG 490) and STAT3 (STAT3 peptide) blocked the increase in BrdUrd<jats:sup>+</jats:sup>cell numbers at 6 h post stimulation with 1 ng/ml IL‐6 (<jats:italic>P</jats:italic> < 0.05). Furthermore, cyclin D1<jats:styled-content style="fixed-case">mRNA</jats:styled-content>expression and cyclin D1<jats:sup>+</jats:sup>/MyoD<jats:sup>+</jats:sup>cell numbers significantly increased in cultures treated with 1 ng/ml IL‐6 compared to those in control media (<jats:italic>P</jats:italic> < 0.05). In contrast, treatment with 10 and 100 ng/ml IL‐6 did not stimulate cell proliferation. Treatment with 10 ng/ml IL‐6 induced greater SOCS3<jats:styled-content style="fixed-case">mRNA</jats:styled-content>expression than with 1 ng/ml IL‐6 and control media. Moreover, co‐localization of SOCS3 and myogenin was observed after treatment with 10 ng/ml IL‐6.</jats:p></jats:sec><jats:sec><jats:title>Conclusions</jats:title><jats:p><jats:styled-content style="fixed-case">IL</jats:styled-content>‐6 induced dose‐dependent increase in satellite cell proliferation by activating the<jats:styled-content style="fixed-case">JAK</jats:styled-content>2/<jats:styled-content style="fixed-case">STAT</jats:styled-content>3/cyclin D1 pathway.</jats:p></jats:sec>
収録刊行物
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- Cell Proliferation
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Cell Proliferation 46 (4), 365-373, 2013-07-21
Wiley
