Protective role of the HOG pathway against the growth defect caused by impaired biosynthesis of complex sphingolipids in yeast <i>Saccharomyces cerevisiae</i>
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- Yutaro Yamaguchi
- Department of Chemistry, Faculty of Sciences Kyushu University, 744, Motooka Nishi‐ku Fukuoka 819‐3905 Japan
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- Yuka Katsuki
- Department of Chemistry, Faculty of Sciences Kyushu University, 744, Motooka Nishi‐ku Fukuoka 819‐3905 Japan
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- Seiya Tanaka
- Department of Chemistry, Faculty of Sciences Kyushu University, 744, Motooka Nishi‐ku Fukuoka 819‐3905 Japan
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- Ryotaro Kawaguchi
- Department of Chemistry, Faculty of Sciences Kyushu University, 744, Motooka Nishi‐ku Fukuoka 819‐3905 Japan
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- Hiroto Denda
- Department of Biofunctional Science and Technology, Graduate School of Biosphere Science Hiroshima University, Kagamiyama 1‐4‐4 Higashi‐Hiroshima 739‐8528 Japan
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- Takuma Ikeda
- Department of Biofunctional Science and Technology, Graduate School of Biosphere Science Hiroshima University, Kagamiyama 1‐4‐4 Higashi‐Hiroshima 739‐8528 Japan
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- Kouichi Funato
- Department of Biofunctional Science and Technology, Graduate School of Biosphere Science Hiroshima University, Kagamiyama 1‐4‐4 Higashi‐Hiroshima 739‐8528 Japan
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- Motohiro Tani
- Department of Chemistry, Faculty of Sciences Kyushu University, 744, Motooka Nishi‐ku Fukuoka 819‐3905 Japan
書誌事項
- 公開日
- 2017-12-22
- 資源種別
- journal article
- 権利情報
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- http://onlinelibrary.wiley.com/termsAndConditions#vor
- DOI
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- 10.1111/mmi.13886
- 公開者
- Wiley
この論文をさがす
説明
<jats:title>Summary</jats:title><jats:p>Complex sphingolipids play critical roles in various cellular events in the yeast <jats:italic>Saccharomyces cerevisiae</jats:italic>. To identify genes that are related to the growth defect caused by disruption of complex sphingolipid biosynthesis, we screened for suppressor mutations and multicopy suppressor genes that confer resistance against repression of <jats:italic>AUR1</jats:italic> encoding inositol phosphorylceramide synthase. From the results of this screening, we found that the activation of high‐osmolarity glycerol (HOG) pathway is involved in suppression of growth defect caused by impaired biosynthesis of complex sphingolipids. Furthermore, it was found that transcriptional regulation via Msn2, Msn4 and Sko1 is involved in the suppressive effect of the HOG pathway. Lack of the HOG pathway did not enhance the reductions in complex sphingolipid levels or the increase in ceramide level caused by the <jats:italic>AUR1</jats:italic> repression, implying that the suppressive effect of the HOG pathway on the growth defect is not attributed to restoration of impaired biosynthesis of complex sphingolipids. On the contrary, the HOG pathway and Msn2/4‐mediated transcriptional activation was involved in suppression of aberrant reactive oxygen species accumulation caused by the <jats:italic>AUR1</jats:italic> repression. These results indicated that the HOG pathway plays pivotal roles in maintaining cell growth under impaired biosynthesis of complex sphingolipids.</jats:p>
収録刊行物
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- Molecular Microbiology
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Molecular Microbiology 107 (3), 363-386, 2017-12-22
Wiley
