Roles of IL-1α/β in regeneration of cardiotoxin-injured muscle and satellite cell function
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- Chayanit Chaweewannakorn
- Division of Advanced Prosthetic Dentistry, Tohoku University Graduate School of Dentistry, Sendai, Japan
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- Masahiro Tsuchiya
- Department of Nursing, Tohoku Fukushi University, Sendai, Japan
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- Masashi Koide
- Department of Orthopaedic Surgery, Tohoku University Graduate School of Medicine, Sendai, Japan
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- Hiroyasu Hatakeyama
- Tohoku University Graduate School of Biomedical Engineering, Sendai, Japan
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- Yukinori Tanaka
- Division of Oral Immunology, Tohoku University Graduate School of Dentistry, Sendai, Japan
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- Shinichirou Yoshida
- Department of Orthopaedic Surgery, Tohoku University Graduate School of Medicine, Sendai, Japan
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- Shunji Sugawara
- Division of Oral Immunology, Tohoku University Graduate School of Dentistry, Sendai, Japan
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- Yoshihiro Hagiwara
- Department of Orthopaedic Surgery, Tohoku University Graduate School of Medicine, Sendai, Japan
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- Keiichi Sasaki
- Division of Advanced Prosthetic Dentistry, Tohoku University Graduate School of Dentistry, Sendai, Japan
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- Makoto Kanzaki
- Tohoku University Graduate School of Biomedical Engineering, Sendai, Japan
書誌事項
- 公開日
- 2018-07-01
- 資源種別
- journal article
- DOI
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- 10.1152/ajpregu.00310.2017
- 公開者
- American Physiological Society
この論文をさがす
説明
<jats:p>Skeletal muscle regeneration after injury is a complex process involving interactions between inflammatory microenvironments and satellite cells. Interleukin (IL)-1 is a key mediator of inflammatory responses and exerts pleiotropic impacts on various cell types. Thus, we aimed to investigate the role of IL-1 during skeletal muscle regeneration. We herein show that IL-1α/β-double knockout (IL-1KO) mice exhibit delayed muscle regeneration after cardiotoxin (CTX) injection, characterized by delayed infiltrations of immune cells accompanied by suppressed local production of proinflammatory factors including IL-6 and delayed increase of paired box 7 (PAX7)-positive satellite cells postinjury compared with those of wild-type (WT) mice. A series of in vitro experiments using satellite cells obtained from the IL-1KO mice unexpectedly revealed that IL-1KO myoblasts have impairments in terms of both proliferation and differentiation, both of which were reversed by exogenous IL-1β administration in culture. Intriguingly, the delay in myogenesis was not attributable to the myogenic transcriptional program since MyoD and myogenin were highly upregulated in IL-1KO cells, instead appearing, at least in part, to be due to dysregulation of cellular fusion events, possibly resulting from aberrant actin regulatory systems. We conclude that IL-1 plays a positive role in muscle regeneration by coordinating the initial interactions among inflammatory microenvironments and satellite cells. Our findings also provide compelling evidence that IL-1 is intimately engaged in regulating the fundamental function of myocytes.</jats:p>
収録刊行物
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- American Journal of Physiology-Regulatory, Integrative and Comparative Physiology
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American Journal of Physiology-Regulatory, Integrative and Comparative Physiology 315 (1), R90-R103, 2018-07-01
American Physiological Society
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キーワード
- Male
- Mice, Inbred BALB C
- Time Factors
- Satellite Cells, Skeletal Muscle
- Interleukin-1beta
- Muscle Development
- Disease Models, Animal
- Muscular Diseases
- Interleukin-1alpha
- Animals
- Regeneration
- Myogenin
- Stem Cell Niche
- Muscle, Skeletal
- Cell Proliferation
- MyoD Protein
- Signal Transduction
- Toxins, Biological
詳細情報 詳細情報について
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- CRID
- 1360004236327207808
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- ISSN
- 15221490
- 03636119
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- PubMed
- 29513560
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- 資料種別
- journal article
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- データソース種別
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- Crossref
- KAKEN
- OpenAIRE