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- Susumu Tanaka
- Department of Anatomy and Cell Science, Kansai Medical University, Hirakata, Osaka 573‑1010, Japan
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- Yoshiko Honda
- SLEEP Disorders Project, Tokyo Metropolitan Institute of Medical Science, Tokyo 156‑8506, Japan
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- Shizuka Takaku
- SLEEP Disorders Project, Tokyo Metropolitan Institute of Medical Science, Tokyo 156‑8506, Japan
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- Taro Koike
- Department of Anatomy and Cell Science, Kansai Medical University, Hirakata, Osaka 573‑1010, Japan
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- Souichi Oe
- Department of Anatomy and Cell Science, Kansai Medical University, Hirakata, Osaka 573‑1010, Japan
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- Yukie Hirahara
- Department of Anatomy and Cell Science, Kansai Medical University, Hirakata, Osaka 573‑1010, Japan
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- Takashi Yoshida
- Department of Urology and Andrology, Kansai Medical University, Hirakata, Osaka 573‑1191, Japan
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- Nae Takizawa
- Department of Anatomy and Cell Science, Kansai Medical University, Hirakata, Osaka 573‑1010, Japan
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- Yasuharu Takamori
- Department of Anatomy and Cell Science, Kansai Medical University, Hirakata, Osaka 573‑1010, Japan
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- Kiyoshi Kurokawa
- Department of Anatomy and Cell Science, Kansai Medical University, Hirakata, Osaka 573‑1010, Japan
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- Tohru Kodama
- SLEEP Disorders Project, Tokyo Metropolitan Institute of Medical Science, Tokyo 156‑8506, Japan
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- Hisao Yamada
- Department of Anatomy and Cell Science, Kansai Medical University, Hirakata, Osaka 573‑1010, Japan
書誌事項
- 公開日
- 2019-03-20
- 資源種別
- journal article
- DOI
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- 10.3892/ijmm.2019.4143
- 10.3892/or.2019.7081
- 公開者
- Spandidos Publications
この論文をさがす
説明
The hypocretin/orexin neuropeptide system coordinates the regulation of various physiological processes. Our previous study reported that a reduction in the expression of pleomorphic adenoma gene‑like 1 (Plagl1), which encodes a C2H2 zinc‑finger transcription factor, occurs in hypocretin neuron‑ablated transgenic mice, suggesting that PLAGL1 is co‑expressed in hypocretin neurons and regulates hypocretin transcription. The present study examined whether canonical prepro‑hypocretin transcription is functionally modulated by PLAGL1. Double immunostaining indicated that the majority of hypocretin neurons were positive for PLAGL1 immunoreactivity in the nucleus. Notably, PLAGL1 immunoreactivity in hypocretin neurons was altered in response to several conditions affecting hypocretin function. An uneven localization of PLAGL1 was detected in the nuclei of hypocretin neurons following sleep deprivation. Chromatin immunoprecipitation revealed that endogenous PLAGL1 may bind to a putative PLAGL1‑binding site in the proximal region of the hypocretin gene, in the murine hypothalamus. In addition, electroporation of the PLAGL1 expression vector into the fetal hypothalamus promoted hypothalamic hypocretin transcription. These results suggested that PLAGL1 may regulate hypothalamic hypocretin transcription.
収録刊行物
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- International Journal of Molecular Medicine
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International Journal of Molecular Medicine 2019-03-20
Spandidos Publications
