Characterization of an Inducible P450 Hydroxylase Involved in the Rice Diterpene Phytoalexin Biosynthetic Pathway
書誌事項
- 公開日
- 1995-02
- 権利情報
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- https://www.elsevier.com/tdm/userlicense/1.0/
- DOI
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- 10.1006/abbi.1995.1094
- 公開者
- Elsevier BV
この論文をさがす
説明
Ent-isopimara-8(14),15-dien-3 beta-ol (1) was isolated from uv-irradiated rice (Oryza sativa L.) leaves. Since 1 was converted to the rice diterpene phytoalexins and oryzalexins D (ent-isopimara-8(14),15-diene-3 beta,7 alpha-diol) and E (ent-isopimara-8(14),15-diene-3 beta,9 alpha-diol) in uv-irradiated rice leaf microsome fraction in the presence of oxygen and NADPH, it was concluded that 1 was the biosynthetic precursor of these oryzalexins. This enzyme reaction was inhibited by cytochrome P450 inhibitors such as piperonyl butoxide, SKF-525A, paclobutorazole, metyrapone, cytochrome c, and carbon monoxide. The lack of inhibitory activity of 1 for spore germination of Pyricularia oryzae indicates that the final cytochrome P450-dependent hydroxylation steps in converting 1 to oryzalexins D and E are essential for the production of rice phytoalexins with antifungal activities.
収録刊行物
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- Archives of Biochemistry and Biophysics
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Archives of Biochemistry and Biophysics 316 (2), 707-712, 1995-02
Elsevier BV