Live tracking of basal stem cells of the epidermis during growth, homeostasis and injury response in zebrafish

DOI PDF PDF 被引用文献1件 参考文献49件
  • Zhengcheng Liu
    , Tokyo Institute of Technology School of Life Science and Technology , 4259 Nagatsuta, Midori-ku, Yokohama 226-8501 , Japan
  • Yidan Meng
    , Tokyo Institute of Technology School of Life Science and Technology , 4259 Nagatsuta, Midori-ku, Yokohama 226-8501 , Japan
  • Atsushi Kawakami
    , Tokyo Institute of Technology School of Life Science and Technology , 4259 Nagatsuta, Midori-ku, Yokohama 226-8501 , Japan
  • Ayu Ishikura
    , Tokyo Institute of Technology School of Life Science and Technology , 4259 Nagatsuta, Midori-ku, Yokohama 226-8501 , Japan

書誌事項

公開日
2024-01-15
資源種別
journal article
権利情報
  • http://www.biologists.com/user-licence-1-1/
DOI
  • 10.1242/dev.202315
公開者
The Company of Biologists

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説明

<jats:title>ABSTRACT</jats:title> <jats:p>Basal stem cells of the epidermis continuously differentiate into keratinocytes and replenish themselves via self-renewal to maintain skin homeostasis. Numerous studies have attempted to reveal how basal cells undergo differentiation or self-renewal; however, this has been hampered by a lack of robust basal cell markers and analytical platforms that allow single-cell tracking. Here, we report that zebrafish integrin beta 4 is a useful marker for basal cell labelling, irrespective of the body region, stage and regenerative status. We employed Cre-loxP recombination in combination with live cell tracking of single basal clones in the caudal fin and investigated the embryonic origin and behaviour of basal cells during fish growth and homeostasis. Although most basal cells, including those in fins, became quiescent in the adult stage, genetic cell ablation showed that basal cells were reactivated to either self-renew or differentiate, depending on the injured cell type. Our study provides a simple and easy-to-use platform for quantitative in vivo imaging of basal stem cells at wider stages and under various conditions.</jats:p>

収録刊行物

  • Development

    Development 151 (2), 2024-01-15

    The Company of Biologists

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