Mitochondrial DNA editing in potato through mitoTALEN and mitoTALECD: molecular characterization and stability of editing events

DOI HANDLE HANDLE PDF Web Site 被引用文献1件 参考文献41件 オープンアクセス

書誌事項

公開日
2024-01-05
資源種別
journal article
権利情報
  • https://creativecommons.org/licenses/by/4.0
  • https://creativecommons.org/licenses/by/4.0
DOI
  • 10.1186/s13007-023-01124-9
公開者
Springer Science and Business Media LLC

説明

<jats:title>Abstract</jats:title><jats:sec> <jats:title>Background</jats:title> <jats:p>The aim of this study was to evaluate and characterize the mutations induced by two TALE-based approaches, double-strand break (DSB) induction by the <jats:italic>Fok</jats:italic>I nuclease (mitoTALEN) and targeted base editing by the DddA cytidine deaminase (mitoTALECD), to edit, for the first time, the mitochondrial genome of potato, a vegetatively propagated crop. The two methods were used to knock out the same mitochondrial target sequence (<jats:italic>orf125</jats:italic>).</jats:p> </jats:sec><jats:sec> <jats:title>Results</jats:title> <jats:p>Targeted chondriome deletions of different sizes (236–1066 bp) were induced by mitoTALEN due to DSB repair through ectopic homologous recombination of short direct repeats (11–12 bp) present in the target region. Furthermore, in one case, the induced DSB and subsequent repair resulted in the amplification of an already present substoichiometric molecule showing a 4288 bp deletion spanning the target sequence. With the mitoTALECD approach, both nonsense and missense mutations could be induced by base substitution. The deletions and single nucleotide mutations were either homoplasmic or heteroplasmic. The former were stably inherited in vegetative offspring.</jats:p> </jats:sec><jats:sec> <jats:title>Conclusions</jats:title> <jats:p>Both editing approaches allowed us to obtain plants with precisely modified mitochondrial genomes at high frequency. The use of the same plant genotype and mtDNA region allowed us to compare the two methods for efficiency, accuracy, type of modifications induced and stability after vegetative propagation.</jats:p> </jats:sec>

収録刊行物

  • Plant Methods

    Plant Methods 20 (1), 2024-01-05

    Springer Science and Business Media LLC

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