A synthetic biology approach to transform <i>Yarrowia lipolytica</i> into a competitive biotechnological producer of β‐carotene

DOI Web Site Web Site 被引用文献1件
  • Macarena Larroude
    BIMLip, Biologie Intégrative du Métabolisme Lipidique Team, Micalis Institute, INRA, AgroParisTech Université Paris‐Saclay Jouy‐en‐Josas France
  • Ewelina Celinska
    Department of Biotechnology and Food Microbiology Poznan University of Life Sciences Poznan Poland
  • Alexandre Back
    BIMLip, Biologie Intégrative du Métabolisme Lipidique Team, Micalis Institute, INRA, AgroParisTech Université Paris‐Saclay Jouy‐en‐Josas France
  • Stephan Thomas
    BIMLip, Biologie Intégrative du Métabolisme Lipidique Team, Micalis Institute, INRA, AgroParisTech Université Paris‐Saclay Jouy‐en‐Josas France
  • Jean‐Marc Nicaud
    BIMLip, Biologie Intégrative du Métabolisme Lipidique Team, Micalis Institute, INRA, AgroParisTech Université Paris‐Saclay Jouy‐en‐Josas France
  • Rodrigo Ledesma‐Amaro
    BIMLip, Biologie Intégrative du Métabolisme Lipidique Team, Micalis Institute, INRA, AgroParisTech Université Paris‐Saclay Jouy‐en‐Josas France

書誌事項

公開日
2017-11-03
権利情報
  • http://onlinelibrary.wiley.com/termsAndConditions#vor
DOI
  • 10.1002/bit.26473
公開者
Wiley

この論文をさがす

説明

<jats:title>Abstract</jats:title><jats:sec><jats:label/><jats:p>The increasing market demands of β‐carotene as colorant, antioxidant and vitamin precursor, requires novel biotechnological production platforms. <jats:italic>Yarrowia lipolytica</jats:italic>, is an industrial organism unable to naturally synthesize carotenoids but with the ability to produce high amounts of the precursor Acetyl‐CoA. We first found that a lipid overproducer strain was capable of producing more β‐carotene than a wild type after expressing the heterologous pathway. Thereafter, we developed a combinatorial synthetic biology approach base on Golden Gate DNA assembly to screen the optimum promoter‐gene pairs for each transcriptional unit expressed. The best strain reached a production titer of 1.5 g/L and a maximum yield of 0.048 g/g of glucose in flask. β‐carotene production was further increased in controlled conditions using a fed‐batch fermentation. A total production of β‐carotene of 6.5 g/L and 90 mg/g DCW with a concomitant production of 42.6 g/L of lipids was achieved. Such high titers suggest that engineered <jats:italic>Y. lipolytica</jats:italic> is a competitive producer organism of β‐carotene.</jats:p></jats:sec>

収録刊行物

被引用文献 (1)*注記

もっと見る

問題の指摘

ページトップへ