Antiribosomal P protein antibody in human systemic lupus erythematosus reacts specifically with activated T cells
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- K Nakanishi
- Department of Internal Medicine, Teikyo University School of Medicine, Tokyo, Japan
書誌事項
- 公開日
- 2001-09
- 権利情報
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- https://journals.sagepub.com/page/policies/text-and-data-mining-license
- DOI
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- 10.1191/096120301682430195
- 公開者
- SAGE Publications
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説明
<jats:p> We examined the presence of the epitope recognized by antiribosomal P protein antibody (anti-P) on the cell surface of human immunocompetent cells. Highly purified CD4/ and CD8/ T cells, and B cells from normal healthy individuals were reacted with affinity-purified IgG anti-P, and were stained with FITC-conjugated F(ab<jats:sup>0</jats:sup>)<jats:sub>2</jats:sub>, fragment goat anti-human IgG, followed by analysis on flow cytometry with gating for viable cells by propidium iodide staining. The presence of an epitope that is antigenically related to the carboxyl-terminal 22-amino-acid sequence of ribosomal P protein was not demonstrated on the surface of fresh CD4/ and CD8/ T cells, or fresh B cells. However, the expression of the ribosomal P epitope was induced on CD4/ and CD8/ T cells after activation with immobilized anti-CD3, whereas the epitope was not expressed on activated B cells. These results indicate that anti-P is an antilymphocyte antibody, which reacts specifically with activated T cells but not with resting T cells or B cells, suggesting possible direct effects of anti-P on the immune dysregulation in systemic lupus erythematosus. </jats:p>
収録刊行物
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- Lupus
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Lupus 10 (9), 612-621, 2001-09
SAGE Publications
