Metabolic profiling analysis of head and neck squamous cell carcinoma

  • Toshimitsu Ohashi
    Department of Otolaryngology‐Head and Neck Surgery Gifu University Graduate School of Medicine Gifu Japan
  • Kosuke Terazawa
    Department of Otolaryngology‐Head and Neck Surgery Gifu University Graduate School of Medicine Gifu Japan
  • Hirofumi Shibata
    Department of Otolaryngology‐Head and Neck Surgery Gifu University Graduate School of Medicine Gifu Japan
  • Norimitsu Inoue
    Department of Molecular Genetics Wakayama Medical University Wakayama Japan
  • Takenori Ogawa
    Department of Otolaryngology‐Head and Neck Surgery Gifu University Graduate School of Medicine Gifu Japan

書誌事項

公開日
2022-11-28
資源種別
journal article
権利情報
  • http://onlinelibrary.wiley.com/termsAndConditions#vor
DOI
  • 10.1111/odi.14432
公開者
Wiley

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説明

<jats:title>Abstract</jats:title><jats:sec><jats:title>Objective</jats:title><jats:p>Tumor cells can acquire a large amount of energy and structural components by reprogramming energy metabolism; moreover, metabolic profiles slightly differ according to cancer type. This study compared and assessed the metabolic profile of head and neck squamous cell carcinoma (HNSCC) and normal tissues, which were collected from patients without cancer.</jats:p></jats:sec><jats:sec><jats:title>Subjects and Methods</jats:title><jats:p>Overall, 23 patients with HNSCC and 6 patients without cancer were included in the analysis. Metabolomic profiles were analyzed using capillary electrophoresis‐mass spectrometry. Gene expression was evaluated using real‐time reverse transcription‐polymerase chain reaction.</jats:p></jats:sec><jats:sec><jats:title>Results</jats:title><jats:p>Glycolysis, the pentose phosphate pathway, tricarboxylic acid cycle, and glutamine metabolism were upregulated in HNSCC tissues based on gene expression analysis. HNSCC could then have enhanced energy production and structural component. The levels of lactate, succinate, glutathione, 2‐hydroxyglutarate, and S‐adenosylmethionine, considered as oncometabolites, increased and these had accumulated in HNSCC tissues.</jats:p></jats:sec><jats:sec><jats:title>Conclusions</jats:title><jats:p>The level of metabolites and the expression of enzymes differ between HNSCC and normal tissues. Reprogramming metabolism in HNSCC provides an energy source as well as structural components, creating a system that offers rapid proliferation, progression, and is less likely to be eliminated.</jats:p></jats:sec>

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