Controlled X‐chromosome dynamics defines meiotic potential of female mouse <i>in vitro</i> germ cells

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  • Jacqueline Severino
    Centre for Genomic Regulation (CRG) The Barcelona Institute of Science and Technology Barcelona Spain
  • Moritz Bauer
    Centre for Genomic Regulation (CRG) The Barcelona Institute of Science and Technology Barcelona Spain
  • Tom Mattimoe
    Centre for Genomic Regulation (CRG) The Barcelona Institute of Science and Technology Barcelona Spain
  • Niccolò Arecco
    Centre for Genomic Regulation (CRG) The Barcelona Institute of Science and Technology Barcelona Spain
  • Luca Cozzuto
    Centre for Genomic Regulation (CRG) The Barcelona Institute of Science and Technology Barcelona Spain
  • Patricia Lorden
    CNAG‐CRG, Centre for Genomic Regulation (CRG) The Barcelona Institute of Science and Technology (BIST) Barcelona Spain
  • Norio Hamada
    Department of Obstetrics and Gynecology Graduate School of Medical Sciences Kyushu University Fukuoka Japan
  • Yoshiaki Nosaka
    Institute for the Advanced Study of Human Biology (ASHBi) Kyoto University Kyoto Japan
  • So I Nagaoka
    Institute for the Advanced Study of Human Biology (ASHBi) Kyoto University Kyoto Japan
  • Pauline Audergon
    Centre for Genomic Regulation (CRG) The Barcelona Institute of Science and Technology Barcelona Spain
  • Antonio Tarruell
    Centre for Genomic Regulation (CRG) The Barcelona Institute of Science and Technology Barcelona Spain
  • Holger Heyn
    CNAG‐CRG, Centre for Genomic Regulation (CRG) The Barcelona Institute of Science and Technology (BIST) Barcelona Spain
  • Katsuhiko Hayashi
    Department of Stem Cell Biology and Medicine Graduate School of Medical Sciences Kyushu University Fukuoka Japan
  • Mitinori Saitou
    Institute for the Advanced Study of Human Biology (ASHBi) Kyoto University Kyoto Japan
  • Bernhard Payer
    Centre for Genomic Regulation (CRG) The Barcelona Institute of Science and Technology Barcelona Spain
  • Hubrecht Institute with UMC

書誌事項

公開日
2022-05-23
資源種別
journal article
権利情報
  • http://creativecommons.org/licenses/by/4.0/
DOI
  • 10.15252/embj.2021109457
公開者
Springer Science and Business Media LLC

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説明

The mammalian germline is characterized by extensive epigenetic reprogramming during its development into functional eggs and sperm. Specifically, the epigenome requires resetting before parental marks can be established and transmitted to the next generation. In the female germline, X-chromosome inactivation and reactivation are among the most prominent epigenetic reprogramming events, yet very little is known about their kinetics and biological function. Here, we investigate X-inactivation and reactivation dynamics using a tailor-made in vitro system of primordial germ cell-like cell (PGCLC) differentiation from mouse embryonic stem cells. We find that X-inactivation in PGCLCs in vitro and in germ cell-competent epiblast cells in vivo is moderate compared to somatic cells, and frequently characterized by escaping genes. X-inactivation is followed by step-wise X-reactivation, which is mostly completed during meiotic prophase I. Furthermore, we find that PGCLCs which fail to undergo X-inactivation or reactivate too rapidly display impaired meiotic potential. Thus, our data reveal fine-tuned X-chromosome remodelling as a critical feature of female germ cell development towards meiosis and oogenesis.

収録刊行物

  • The EMBO Journal

    The EMBO Journal 41 (12), 2022-05-23

    Springer Science and Business Media LLC

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参考文献 (86)*注記

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