In-Depth Proteome Analysis of Arabidopsis Leaf Peroxisomes Combined with in Vivo Subcellular Targeting Verification Indicates Novel Metabolic and Regulatory Functions of Peroxisomes

  • Sigrun Reumann
    Michigan State University-Department of Energy Plant Research Laboratory (S.R., S.Q., K.A., P.Y., K.M.-S., R.S., J.H.), Plant Biology Department (K.A., N.L., C.G.W., A.P.M.W., J.H.), and Biochemistry and Molecular Biology Department (C.G.W.), Michigan State University, East Lansing, Michigan 48824; and Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor
  • Sheng Quan
    Michigan State University-Department of Energy Plant Research Laboratory (S.R., S.Q., K.A., P.Y., K.M.-S., R.S., J.H.), Plant Biology Department (K.A., N.L., C.G.W., A.P.M.W., J.H.), and Biochemistry and Molecular Biology Department (C.G.W.), Michigan State University, East Lansing, Michigan 48824; and Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor
  • Kyaw Aung
    Michigan State University-Department of Energy Plant Research Laboratory (S.R., S.Q., K.A., P.Y., K.M.-S., R.S., J.H.), Plant Biology Department (K.A., N.L., C.G.W., A.P.M.W., J.H.), and Biochemistry and Molecular Biology Department (C.G.W.), Michigan State University, East Lansing, Michigan 48824; and Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor
  • Pingfang Yang
    Michigan State University-Department of Energy Plant Research Laboratory (S.R., S.Q., K.A., P.Y., K.M.-S., R.S., J.H.), Plant Biology Department (K.A., N.L., C.G.W., A.P.M.W., J.H.), and Biochemistry and Molecular Biology Department (C.G.W.), Michigan State University, East Lansing, Michigan 48824; and Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor
  • Kalpana Manandhar-Shrestha
    Michigan State University-Department of Energy Plant Research Laboratory (S.R., S.Q., K.A., P.Y., K.M.-S., R.S., J.H.), Plant Biology Department (K.A., N.L., C.G.W., A.P.M.W., J.H.), and Biochemistry and Molecular Biology Department (C.G.W.), Michigan State University, East Lansing, Michigan 48824; and Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor
  • Danielle Holbrook
    Michigan State University-Department of Energy Plant Research Laboratory (S.R., S.Q., K.A., P.Y., K.M.-S., R.S., J.H.), Plant Biology Department (K.A., N.L., C.G.W., A.P.M.W., J.H.), and Biochemistry and Molecular Biology Department (C.G.W.), Michigan State University, East Lansing, Michigan 48824; and Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor
  • Nicole Linka
    Michigan State University-Department of Energy Plant Research Laboratory (S.R., S.Q., K.A., P.Y., K.M.-S., R.S., J.H.), Plant Biology Department (K.A., N.L., C.G.W., A.P.M.W., J.H.), and Biochemistry and Molecular Biology Department (C.G.W.), Michigan State University, East Lansing, Michigan 48824; and Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor
  • Robert Switzenberg
    Michigan State University-Department of Energy Plant Research Laboratory (S.R., S.Q., K.A., P.Y., K.M.-S., R.S., J.H.), Plant Biology Department (K.A., N.L., C.G.W., A.P.M.W., J.H.), and Biochemistry and Molecular Biology Department (C.G.W.), Michigan State University, East Lansing, Michigan 48824; and Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor
  • Curtis G. Wilkerson
    Michigan State University-Department of Energy Plant Research Laboratory (S.R., S.Q., K.A., P.Y., K.M.-S., R.S., J.H.), Plant Biology Department (K.A., N.L., C.G.W., A.P.M.W., J.H.), and Biochemistry and Molecular Biology Department (C.G.W.), Michigan State University, East Lansing, Michigan 48824; and Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor
  • Andreas P.M. Weber
    Michigan State University-Department of Energy Plant Research Laboratory (S.R., S.Q., K.A., P.Y., K.M.-S., R.S., J.H.), Plant Biology Department (K.A., N.L., C.G.W., A.P.M.W., J.H.), and Biochemistry and Molecular Biology Department (C.G.W.), Michigan State University, East Lansing, Michigan 48824; and Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor
  • Laura J. Olsen
    Michigan State University-Department of Energy Plant Research Laboratory (S.R., S.Q., K.A., P.Y., K.M.-S., R.S., J.H.), Plant Biology Department (K.A., N.L., C.G.W., A.P.M.W., J.H.), and Biochemistry and Molecular Biology Department (C.G.W.), Michigan State University, East Lansing, Michigan 48824; and Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor
  • Jianping Hu
    Michigan State University-Department of Energy Plant Research Laboratory (S.R., S.Q., K.A., P.Y., K.M.-S., R.S., J.H.), Plant Biology Department (K.A., N.L., C.G.W., A.P.M.W., J.H.), and Biochemistry and Molecular Biology Department (C.G.W.), Michigan State University, East Lansing, Michigan 48824; and Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor

抄録

<jats:title>Abstract</jats:title><jats:p>Peroxisomes are metabolically diverse organelles with essential roles in plant development. The major protein constituents of plant peroxisomes are well characterized, whereas only a few low-abundance and regulatory proteins have been reported to date. We performed an in-depth proteome analysis of Arabidopsis (Arabidopsis thaliana) leaf peroxisomes using one-dimensional gel electrophoresis followed by liquid chromatography and tandem mass spectrometry. We detected 65 established plant peroxisomal proteins, 30 proteins whose association with Arabidopsis peroxisomes had been previously demonstrated only by proteomic data, and 55 putative novel proteins of peroxisomes. We subsequently tested the subcellular targeting of yellow fluorescent protein fusions for selected proteins and confirmed the peroxisomal localization for 12 proteins containing predicted peroxisome targeting signals type 1 or 2 (PTS1/2), three proteins carrying PTS-related peptides, and four proteins that lack conventional targeting signals. We thereby established the tripeptides SLM&gt; and SKV&gt; (where &gt; indicates the stop codon) as new PTS1s and the nonapeptide RVx5HF as a putative new PTS2. The 19 peroxisomal proteins conclusively identified from this study potentially carry out novel metabolic and regulatory functions of peroxisomes. Thus, this study represents an important step toward defining the complete plant peroxisomal proteome.</jats:p>

収録刊行物

  • Plant Physiology

    Plant Physiology 150 (1), 125-143, 2009-03-27

    Oxford University Press (OUP)

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