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Single-strand conformation polymorphism analysis
Description
<jats:title>Abstract</jats:title> <jats:p>SSCP (single-strand conformation polymorphism) is widely used for mutation detection because of its simplicity and versatility. In this method, a segment to be searched for a mutation is amplified by PCR from genomic DNA or cDNA, denatured, and separated by electrophoresis in media having sieving properties without denaturant (1). Mutation usually causes a mobility shift in electrophoresis, and so, is detected. Conformational change of the single stranded mutant DNA is believed to be the reason for the mobility shift.</jats:p>
Journal
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- Mutation Detection
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Mutation Detection 7-24, 1998-02-05
Oxford University PressOxford
