Therapeutic effects of a recombinant human collagen peptide bioscaffold with human adipose-derived stem cells on impaired wound healing after radiotherapy
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- Takanobu Mashiko
- Department of Plastic Surgery; Jichi Medical University; Tochigi Japan
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- Hitomi Takada
- Stem Cell Technologies lab, Graduate School of Biological Sciences; Nara Institute of Science and Technology; Nara Japan
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- Szu-Hsien Wu
- Department of Plastic Surgery; University of Tokyo, School of Medicine; Tokyo Japan
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- Koji Kanayama
- Department of Plastic Surgery; Jichi Medical University; Tochigi Japan
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- Jingwei Feng
- Department of Plastic Surgery; University of Tokyo, School of Medicine; Tokyo Japan
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- Kensuke Tashiro
- Department of Plastic Surgery; University of Tokyo, School of Medicine; Tokyo Japan
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- Rintaro Asahi
- Department of Plastic Surgery; Jichi Medical University; Tochigi Japan
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- Ataru Sunaga
- Department of Plastic Surgery; Jichi Medical University; Tochigi Japan
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- Kazuto Hoshi
- Department of Oral Surgery; University of Tokyo, School of Medicine; Tokyo Japan
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- Akira Kurisaki
- Stem Cell Technologies lab, Graduate School of Biological Sciences; Nara Institute of Science and Technology; Nara Japan
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- Tsuyoshi Takato
- Department of Oral Surgery; University of Tokyo, School of Medicine; Tokyo Japan
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- Kotaro Yoshimura
- Department of Plastic Surgery; Jichi Medical University; Tochigi Japan
書誌事項
- 公開日
- 2018-03-04
- 資源種別
- journal article
- 権利情報
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- http://doi.wiley.com/10.1002/tdm_license_1.1
- http://onlinelibrary.wiley.com/termsAndConditions#vor
- DOI
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- 10.1002/term.2647
- 公開者
- Wiley
この論文をさがす
説明
Chronic changes following radiotherapy include alterations in tissue-resident stem cells and vasculatures, which can lead to impaired wound healing. In this study, novel recombinant human collagen peptide (rhCP) scaffolds were evaluated as a biomaterial carrier for cellular regenerative therapy. Human adipose-derived stem cells (hASCs) were successfully cultured on rhCP scaffolds. By hASC culture on rhCP, microarray assay indicated that expression of genes related to cell proliferation and extracellular matrix production was upregulated. Pathway analyses revealed that signaling pathways related to inflammatory suppression and cell growth promotion were activated as well as signaling pathways consistent with some growth factors including vascular endothelial growth factor, hepatocyte growth factor, and transforming growth factor beta, although gene expression of these growth factors was not upregulated. These findings suggest the rhCP scaffold showed similar biological actions to cytokines regulating cell growth and immunity. In subsequent impaired wound healing experiments using a locally irradiated (20 Gray) mouse, wound treatment with rhCP sponges combined with cultured hASCs and human umbilical vein endothelial cells accelerated wound closure compared with wounds treated with rhCP with hASCs alone, rhCP only, and control (dressing alone), with better healing observed according to this order. These results indicating the therapeutic value of rhCP scaffolds as a topical biomaterial dressing and a biocarrier of stem cells and vascular endothelial cells for regenerating therapies. The combination of rhCP and functional cells was suggested to be a potential tool for revitalizing stem cell-depleted conditions such as radiation tissue damage.
収録刊行物
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- Journal of Tissue Engineering and Regenerative Medicine
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Journal of Tissue Engineering and Regenerative Medicine 12 (5), 1186-1194, 2018-03-04
Wiley
