An In Vitro Three-Dimensional Organotypic Model to Analyze Peripancreatic Fat Invasion in Pancreatic Cancer: A Culture System Based on Collagen Gel Embedding
書誌事項
- 公開日
- 2018-10-31
- 資源種別
- journal article
- 権利情報
-
- http://www.springer.com/tdm
- DOI
-
- 10.1007/978-1-4939-8879-2_11
- 公開者
- Springer New York
この論文をさがす
説明
Three-dimensional culture systems reflect biological environments better than conventional two-dimensional culture. Additionally, three-dimensional culture is a strong experimental tool to analyze direct interactions between cancer cells and stromal cells in vitro. Herein, we describe protocols for an organotypic fat invasion model that is a novel culturing system mimicking the extrapancreatic invasion of pancreatic adenocarcinoma (PDAC). This novel model is based on the collagen I gel embedding method and enables us to analyze the functional and histological interactions between cancer cells and adipose tissue.
収録刊行物
-
- Methods in Molecular Biology
-
Methods in Molecular Biology 135-141, 2018-10-31
Springer New York
