<i>Ex vivo</i> bupivacaine treatment results in increased adipogenesis of skeletal muscle cells in the rat
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- Keitaro Yamanouchi
- Department of Veterinary Physiology Graduate School of Agricultural and Life Sciences The University of Tokyo Bunkyo Tokyo Japan
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- Katsuyuki Nakamura
- Department of Veterinary Physiology Graduate School of Agricultural and Life Sciences The University of Tokyo Bunkyo Tokyo Japan
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- Yuki Takegahara
- Department of Veterinary Physiology Graduate School of Agricultural and Life Sciences The University of Tokyo Bunkyo Tokyo Japan
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- Shin‐ichi Nakano
- Department of Veterinary Physiology Graduate School of Agricultural and Life Sciences The University of Tokyo Bunkyo Tokyo Japan
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- Masugi Nishihara
- Department of Veterinary Physiology Graduate School of Agricultural and Life Sciences The University of Tokyo Bunkyo Tokyo Japan
書誌事項
- 公開日
- 2013-10-03
- 資源種別
- journal article
- 権利情報
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- http://onlinelibrary.wiley.com/termsAndConditions#vor
- DOI
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- 10.1111/asj.12112
- 公開者
- Wiley
この論文をさがす
説明
<jats:title>Abstract</jats:title><jats:p>Intramuscular adipose tissue (<jats:styled-content style="fixed-case">IMAT</jats:styled-content>) is observed in some skeletal muscle pathologies. <jats:styled-content style="fixed-case">IMAT</jats:styled-content> is implicated not only in the disorders of muscle contraction, but also of metabolism and insulin sensitivity due to its nature as a secretary organ. Several studies indicate the presence of cells with adipogenic potential in skeletal muscle. However, the mechanism of fate specification that triggers these cells to enter an adipogenic program <jats:italic>in vivo</jats:italic> remains to be solved. In the present study, we examined whether activation of the adipogenic program of muscle‐resident cells precedes their proliferation upon muscle injury. For this purpose, muscle injury was induced by injecting bupivacaine (<jats:styled-content style="fixed-case">BPVC</jats:styled-content>) to excised skeletal muscle <jats:italic>ex vivo</jats:italic>. Cells isolated from <jats:italic>ex vivo</jats:italic> <jats:styled-content style="fixed-case">BPVC</jats:styled-content>‐treated muscle exhibited higher adipogenic potential than those from saline‐treated muscle. Pre‐plating exposure of skeletal muscle cells to basic fibroblast growth factor (<jats:styled-content style="fixed-case">bFGF</jats:styled-content>) mimicked the effect of <jats:italic>ex vivo</jats:italic> <jats:styled-content style="fixed-case">BPVC</jats:styled-content>‐treatment, suggesting that <jats:styled-content style="fixed-case">bFGF</jats:styled-content> released from extracellular matrix in response to muscle injury activates their adipogenic program. Interestingly, the number of myotubes were significantly reduced in the culture from <jats:styled-content style="fixed-case">BPVC</jats:styled-content>‐treated muscle, suggesting that adipocytes negatively regulate myogenesis.</jats:p>
収録刊行物
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- Animal Science Journal
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Animal Science Journal 84 (11), 757-763, 2013-10-03
Wiley