Coexpressed subunits of dual genetic origin define a conserved supercomplex mediating essential protein import into chloroplasts
-
- Silvia Ramundo
- Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94143;
-
- Yukari Asakura
- Laboratory of Organelle Biology, Institute for Protein Research, Osaka University, Osaka 565-0871, Japan;
-
- Patrice A. Salomé
- Department of Chemistry and Biochemistry, University of California, Los Angeles, CA 90095;
-
- Daniela Strenkert
- Department of Chemistry and Biochemistry, University of California, Los Angeles, CA 90095;
-
- Morgane Boone
- Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94143;
-
- Luke C. M. Mackinder
- Department of Biology, University of York, York YO10 5DD, United Kingdom;
-
- Kazuaki Takafuji
- Graduate School of Medicine, Osaka University, Osaka 565-0871, Japan;
-
- Emine Dinc
- Department of Molecular Biology, University of Geneva, Geneva CH-1211, Switzerland;
-
- Michèle Rahire
- Department of Molecular Biology, University of Geneva, Geneva CH-1211, Switzerland;
-
- Michèle Crèvecoeur
- Department of Molecular Biology, University of Geneva, Geneva CH-1211, Switzerland;
-
- Leonardo Magneschi
- Institute of Plant Biology and Biotechnology, University of Münster, Münster 48143, Germany;
-
- Olivier Schaad
- Department of Biochemistry, University of Geneva, Geneva CH-1211, Switzerland;
-
- Michael Hippler
- Institute of Plant Biology and Biotechnology, University of Münster, Münster 48143, Germany;
-
- Martin C. Jonikas
- Howard Hughes Medical Institute, Chevy Chase, MD 20815;
-
- Sabeeha Merchant
- Department of Chemistry and Biochemistry, University of California, Los Angeles, CA 90095;
-
- Masato Nakai
- Laboratory of Organelle Biology, Institute for Protein Research, Osaka University, Osaka 565-0871, Japan;
-
- Jean-David Rochaix
- Department of Molecular Biology, University of Geneva, Geneva CH-1211, Switzerland;
-
- Peter Walter
- Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94143;
説明
<jats:title>Significance</jats:title> <jats:p> Chloroplasts are of vital importance in photosynthetic eukaryotic organisms. Like mitochondria, they contain their own genomes. Nevertheless, most chloroplast proteins are encoded by nuclear genes, translated in the cytosol, and must cross the chloroplast envelope membranes to reach their proper destinations inside the organelle. Despite its fundamental role, our knowledge of the machinery catalyzing chloroplast protein import is incomplete and controversial. Here, we address the evolutionary conservation, composition, and function of the chloroplast protein import machinery using the green alga <jats:italic>Chlamydomonas reinhardtii</jats:italic> as a model system. Our findings help clarify the current debate regarding the composition of the chloroplast protein import machinery, provide evidence for cross-compartmental coordination of its biogenesis, and open promising avenues for its structural characterization. </jats:p>
収録刊行物
-
- Proceedings of the National Academy of Sciences
-
Proceedings of the National Academy of Sciences 117 (51), 32739-32749, 2020-12-03
Proceedings of the National Academy of Sciences
- Tweet
キーワード
- Chloroplasts
- Evolution
- Sequence Homology
- 612
- Evolution, Molecular
- gene coexpression
- Gene Expression Regulation, Plant
- chloroplast protein import
- Genetics
- Plant Proteins
- chloroplast gene targeting
- Sequence Homology, Amino Acid
- Molecular
- Plant
- Cell Compartmentation
- Amino Acid
- Protein Transport
- Gene Expression Regulation
- Multiprotein Complexes
- Generic health relevance
- Chlamydomonas reinhardtii
詳細情報 詳細情報について
-
- CRID
- 1360853567405958272
-
- ISSN
- 10916490
- 00278424
-
- PubMed
- 33273113
-
- データソース種別
-
- Crossref
- KAKEN
- OpenAIRE
