Regulation of Msh4-Msh5 association with meiotic chromosomes in budding yeast

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  • Krishnaprasad G Nandanan
    School of Biology, Indian Institute of Science Education and Research Thiruvananthapuram, Trivandrum 695016, India
  • Sagar Salim
    School of Biology, Indian Institute of Science Education and Research Thiruvananthapuram, Trivandrum 695016, India
  • Ajith V Pankajam
    School of Biology, Indian Institute of Science Education and Research Thiruvananthapuram, Trivandrum 695016, India
  • Miki Shinohara
    Graduate School of Agriculture, Kindai University, Nara 631-8505, Japan
  • Gen Lin
    Genome Biology Unit, European Molecular Biology Laboratory, Heidelberg 69117, Germany
  • Parijat Chakraborty
    School of Biology, Indian Institute of Science Education and Research Thiruvananthapuram, Trivandrum 695016, India
  • Amamah Farnaz
    School of Biology, Indian Institute of Science Education and Research Thiruvananthapuram, Trivandrum 695016, India
  • Lars M Steinmetz
    Genome Biology Unit, European Molecular Biology Laboratory, Heidelberg 69117, Germany
  • Akira Shinohara
    Institute for Protein Research, Osaka University, Osaka 565-0871, Japan
  • Koodali T Nishant
    School of Biology, Indian Institute of Science Education and Research Thiruvananthapuram, Trivandrum 695016, India

抄録

<jats:title>Abstract</jats:title> <jats:p>In the baker’s yeast Saccharomyces cerevisiae, most of the meiotic crossovers are generated through a pathway involving the highly conserved mismatch repair related Msh4-Msh5 complex. To understand the role of Msh4-Msh5 in meiotic crossing over, we determined its genome wide in vivo binding sites in meiotic cells. We show that Msh5 specifically associates with DSB hotspots, chromosome axes, and centromeres on chromosomes. A basal level of Msh5 association with these chromosomal features is observed even in the absence of DSB formation (spo11Δ mutant) at the early stages of meiosis. But efficient binding to DSB hotspots and chromosome axes requires DSB formation and resection and is enhanced by double Holliday junction structures. Msh5 binding is also correlated to DSB frequency and enhanced on small chromosomes with higher DSB and crossover density. The axis protein Red1 is required for Msh5 association with the chromosome axes and DSB hotspots but not centromeres. Although binding sites of Msh5 and other pro-crossover factors like Zip3 show extensive overlap, Msh5 associates with centromeres independent of Zip3. These results on Msh5 localization in wild type and meiotic mutants have implications for how Msh4-Msh5 works with other pro-crossover factors to ensure crossover formation.</jats:p>

収録刊行物

  • Genetics

    Genetics 219 (2), 2021-07-08

    Oxford University Press (OUP)

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