Baculovirus-Expressed Constructs Induce Immunoglobulin G That Recognizes VAR2CSA on<i>Plasmodium falciparum</i>- Infected Erythrocytes
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- Lea Barfod
- Centre for Medical Parasitology at Copenhagen University Hospital (Rigshospitalet) and Institute for Medical Microbiology and Immunology, University of Copenhagen, Copenhagen, Denmark
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- Morten A. Nielsen
- Centre for Medical Parasitology at Copenhagen University Hospital (Rigshospitalet) and Institute for Medical Microbiology and Immunology, University of Copenhagen, Copenhagen, Denmark
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- Louise Turner
- Centre for Medical Parasitology at Copenhagen University Hospital (Rigshospitalet) and Institute for Medical Microbiology and Immunology, University of Copenhagen, Copenhagen, Denmark
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- Madeleine Dahlbäck
- Centre for Medical Parasitology at Copenhagen University Hospital (Rigshospitalet) and Institute for Medical Microbiology and Immunology, University of Copenhagen, Copenhagen, Denmark
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- Anja T. R. Jensen
- Centre for Medical Parasitology at Copenhagen University Hospital (Rigshospitalet) and Institute for Medical Microbiology and Immunology, University of Copenhagen, Copenhagen, Denmark
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- Lars Hviid
- Centre for Medical Parasitology at Copenhagen University Hospital (Rigshospitalet) and Institute for Medical Microbiology and Immunology, University of Copenhagen, Copenhagen, Denmark
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- Thor G. Theander
- Centre for Medical Parasitology at Copenhagen University Hospital (Rigshospitalet) and Institute for Medical Microbiology and Immunology, University of Copenhagen, Copenhagen, Denmark
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- Ali Salanti
- Centre for Medical Parasitology at Copenhagen University Hospital (Rigshospitalet) and Institute for Medical Microbiology and Immunology, University of Copenhagen, Copenhagen, Denmark
説明
<jats:title>ABSTRACT</jats:title><jats:p>We raised specific antisera against recombinant VAR2CSA domains produced in<jats:italic>Escherichia coli</jats:italic>and in insect cells. All were reactive in enzyme-linked immunosorbent assay, but only insect cell-derived constructs induced immunoglobulin G (IgG) that was reactive with native VAR2CSA on the surface of infected erythrocytes. Our data show that five of the six VAR2CSA Duffy-binding-like domains are surface exposed and that induction of surface-reactive VAR2CSA-specific IgG depends critically upon antigen conformation. These findings have implications for the development of vaccines against pregnancy-associated<jats:italic>Plasmodium falciparum</jats:italic>malaria.</jats:p>
収録刊行物
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- Infection and Immunity
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Infection and Immunity 74 (7), 4357-4360, 2006-07
American Society for Microbiology
