Electrochemical L-Lactic Acid Sensor Based on Immobilized ZnO Nanorods with Lactate Oxidase

Zafar Hussain Ibupoto, Syed Muhammad Usman Ali Shah, Magnus Willander, Kimleang Khun

doi:10.3390/s120302456

Electrochemical L-Lactic Acid Sensor Based on Immobilized ZnO Nanorods with Lactate Oxidase

DOI Web Site 被引用文献2件

Zafar Hussain Ibupoto

Department of Science and Technology, Campus Norrköping, Linköping University, SE-60174, Norrköping, Sweden
Syed Muhammad Usman Ali Shah

Department of Science and Technology, Campus Norrköping, Linköping University, SE-60174, Norrköping, Sweden
Magnus Willander

Department of Science and Technology, Campus Norrköping, Linköping University, SE-60174, Norrköping, Sweden
Kimleang Khun

Department of Science and Technology, Campus Norrköping, Linköping University, SE-60174, Norrköping, Sweden

書誌事項

公開日: 2012-02-23

権利情報

https://creativecommons.org/licenses/by/3.0/

DOI

10.3390/s120302456

公開者: MDPI AG

説明

<jats:p>In this work, fabrication of gold coated glass substrate, growth of ZnO nanorods and potentiometric response of lactic acid are explained. The biosensor was developed by immobilizing the lactate oxidase on the ZnO nanorods in combination with glutaraldehyde as a cross linker for lactate oxidase enzyme. The potentiometric technique was applied for the measuring the output (EMF) response of L-lactic acid biosensor. We noticed that the present biosensor has wide linear detection range of concentration from 1 × 10−4–1 × 100 mM with acceptable sensitivity about 41.33 ± 1.58 mV/decade. In addition, the proposed biosensor showed fast response time less than 10 s, a good selectivity towards L-lactic acid in presence of common interfering substances such as ascorbic acid, urea, glucose, galactose, magnesium ions and calcium ions. The present biosensor based on immobilized ZnO nanorods with lactate oxidase sustained its stability for more than three weeks.</jats:p>