Microbial methane production in deep aquifer associated with the accretionary prism in Southwest Japan

DOI PDF PDF Web Site PubMed 被引用文献13件 オープンアクセス
  • Hiroyuki Kimura
    Department of Geosciences, Faculty of Science, Shizuoka University , Shizuoka , Japan
  • Hiroaki Nashimoto
    Department of Geosciences, Faculty of Science, Shizuoka University , Shizuoka , Japan
  • Mikio Shimizu
    Department of Geosciences, Faculty of Science, Shizuoka University , Shizuoka , Japan
  • Shohei Hattori
    Department of Environmental Science and Technology, Interdisciplinary Graduate School of Science and Engineering, Tokyo Institute of Technology , Yokohama , Japan
  • Keita Yamada
    Department of Environmental Chemistry and Engineering, Interdisciplinary Graduate School of Science and Engineering, Tokyo Institute of Technology , Yokohama , Japan
  • Keisuke Koba
    Faculty of Agriculture, Tokyo University of Agriculture and Technology , Tokyo , Japan
  • Naohiro Yoshida
    Department of Environmental Science and Technology, Interdisciplinary Graduate School of Science and Engineering, Tokyo Institute of Technology , Yokohama , Japan
  • Kenji Kato
    Department of Geosciences, Faculty of Science, Shizuoka University , Shizuoka , Japan

書誌事項

公開日
2009-12-03
権利情報
  • https://academic.oup.com/pages/standard-publication-reuse-rights
DOI
  • 10.1038/ismej.2009.132
公開者
Oxford University Press (OUP)

この論文をさがす

説明

<jats:title>Abstract</jats:title><jats:p>To identify the methanogenic pathways present in a deep aquifer associated with an accretionary prism in Southwest Japan, a series of geochemical and microbiological studies of natural gas and groundwater derived from a deep aquifer were performed. Stable carbon isotopic analysis of methane in the natural gas and dissolved inorganic carbon (mainly bicarbonate) in groundwater suggested that the methane was derived from both thermogenic and biogenic processes. Archaeal 16S rRNA gene analysis revealed the dominance of H2-using methanogens in the groundwater. Furthermore, the high potential of methane production by H2-using methanogens was shown in enrichments using groundwater amended with H2 and CO2. Bacterial 16S rRNA gene analysis showed that fermentative bacteria inhabited the deep aquifer. Anaerobic incubations using groundwater amended with organic substrates and bromoethanesulfonate (a methanogen inhibitor) suggested a high potential of H2 and CO2 generation by fermentative bacteria. To confirm whether or not methane is produced by a syntrophic consortium of H2-producing fermentative bacteria and H2-using methanogens, anaerobic incubations using the groundwater amended with organic substrates were performed. Consequently, H2 accumulation and rapid methane production were observed in these enrichments incubated at 55 and 65 °C. Thus, our results suggested that past and ongoing syntrophic biodegradation of organic compounds by H2-producing fermentative bacteria and H2-using methanogens, as well as a thermogenic reaction, contributes to the significant methane reserves in the deep aquifer associated with the accretionary prism in Southwest Japan.</jats:p>

収録刊行物

  • The ISME Journal

    The ISME Journal 4 (4), 531-541, 2009-12-03

    Oxford University Press (OUP)

被引用文献 (13)*注記

もっと見る

キーワード

問題の指摘

ページトップへ